A QUANTITATIVE ASSESSMENT OF MICROVESSEL ULTRASTRUCTURE IN C-6 ASTROCYTOMA SPHEROIDS TRANSPLANTED TO BRAIN AND TO MUSCLE
A QUANTITATIVE ASSESSMENT OF MICROVESSEL ULTRASTRUCTURE IN C-6 ASTROCYTOMA SPHEROIDS TRANSPLANTED TO BRAIN AND TO MUSCLE
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DOI：
10.1097/00005072-198801000-00004
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发表时间：
1988-01-01
影响因子：
3.2
通讯作者：
DELMAESTRO, RF
中科院分区：
文献类型：
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作者：
COOMBER, BL;STEWART, PA;DELMAESTRO, RF
侵入生长中的肿瘤的正常血管在形态上发生了巨大的变化。血管特征是否完全由肿瘤或肿瘤血管起源的正常血管的发育限制所决定尚不清楚。为了解决这个问题，我们挑战了两种形态不同类型的毛细血管（脑和肌肉）与相同的肿瘤环境（C6星形细胞瘤），并量化了入侵的血管形态。将C6星形细胞瘤细胞的血管球单独植入大鼠大脑皮质或髂肌内。对肿瘤、瘤周组织和对照组织的微血管进行超微结构检查和定量。无论植入部位如何，肿瘤血管与宿主血管显著不同，但彼此之间无差异。肿瘤血管相对于正常宿主血管壁较厚，线粒体和囊泡结构密度低，并且具有高渗透性血管的开窗和扩大的连接裂隙特征。对照脑血管通常壁薄，线粒体密度高，内皮囊泡密度低，紧密连接连续。对照组肌血管壁薄，线粒体密度低，囊泡密度高，交界区偶见裂隙扩大。瘤周血管形态介于肿瘤和相应对照组织之间。我们认为，C6星形细胞瘤细胞影响入侵的内皮细胞发展一种完全不同于宿主组织内皮的渗透性表型，而宿主血管表型不影响肿瘤血管形态。
Normal blood vessels invading a growing neoplasm undergo dramatic changes in morphology. Whether vessel characteristics are dicated entirely by the tumor, or from developmental restrictions in normal vessels from which tumor vessels originate is not known. To address this question we challenged two morphologically different types of capillaries (brain and muscle) with the same tumor environment (C6 astrocytoma), and quantified the invading vessel morphology. A vascular spheroids of C6 astrocytoma cells were implanted singly into rat cerebral cortex or iliacus muscle. Microvessels from the tumor, peritumoral tissue and control tissue were examined ultrastructurally and quantified. Tumor vessels differed significantly from host vessels but not from each other, regardless of implantation site. Neoplastic vessels were thick-walled relative to normal host vessels, had low densities of mitochondria and vesicular structures, and had both fenestrations and enlarged junctional clefts characteristic of highly permeable vessels. Control brain vessels were typically thin-walled, had a high density of mitochondria, a low density of endothelial vesicles and continuous tight junctions. Control muscle vessels were thin-walled with a low density of mitochrondria, high density of vesicles and junctional zones with occasional enlarged clefts. Peritumoral vessel morphology was intermediate between that of tumor and the corresponding control tissue. We propose that C6 astrocytoma cells influences invading endothelial cells to develop a permeable phenotype radically different from host tissue endothelium, and host vessel phenotype does not infuence tumor vessel morphology.