Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana
Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana
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DOI：
10.1007/bf00049339
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发表时间：
1996-02-01
影响因子：
5.1
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Katagiri, T;Mizoguchi, T;Shinozaki, K
二酰基甘油激酶（DGK）从二酰基甘油（蛋白激酶C（PKC）的激活剂）合成磷脂酸以再合成磷脂酰肌醇。DGK的结构尚未在植物中表征。我们报告的cDNA克隆，cATDGK1，编码DGK从拟南芥。cATDGK1 cDNA包含一个2184 bp的开放阅读框，编码728个氨基酸，分子量为79.4 kDa。推导的ATDGK1氨基酸序列与大鼠、猪和果蝇DGKs的氨基酸序列具有显著的相似性。ATDGK1 mRNA在根、茎和叶中均有表达。Southern杂交分析表明ATDGK1基因为单拷贝基因。DGK和磷脂酶C的存在表明植物中存在PKC。
Diacylglycerol kinase (DGK) synthesizes phosphatidic acid from diacylglycerol, an activator of protein kinase C (PKC), to resynthesize phosphatidylinositols. The structure of DGK has not been characterized in plants. We report the cloning of a cDNA, cATDGK1, encoding DGK from Arabidopsis thaliana. The cATDGK1 cDNA contains an open reading frame of 2184 bp, and encodes a putative protein of 728 amino acids with a predicted molecular mass of 79.4 kDa. The deduced ATDGK1 amino acid sequence exhibits significant similarity to that of rat, pig, and Drosophila DGKs. The ATDGK1 mRNA was detected in roots, shoots, and leaves. Southern blot analysis suggests that the ATDGK1 gene is a single-copy gene. The existence of DGK as well as phospholipase C suggests the existence of PKC in plants.