The effect of salidroside on cell damage induced by glutamate and intracellular free calcium in PC12 cells
The effect of salidroside on cell damage induced by glutamate and intracellular free calcium in PC12 cells
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DOI：
10.1080/1028602042000325645
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Wang, Min-Wei
中科院分区：
文献类型：
--
作者：
Cao, Li-Li;Du, Guan-Hua;Wang, Min-Wei
红景天苷（Salidroside，Sald）是从传统中药红景天中提取的一种有效成分，具有抗衰老、抗肿瘤、抗氧化等作用。本实验中，红景天苷对2 mmol/L谷氨酸作用15 min后加入无血清培养液培养24 h的PC 12细胞具有保护作用，其类似于兴奋毒素的体内系统。红景天苷能降低无镁Hanks液和D-Hanks液中PC 12细胞的[Ca ~（2+）]i，但对Hanks液中的基础[Ca ~（2+）]i无影响。红景天苷还能抑制KCl和谷氨酸引起的[Ca ~（2+）]i升高。综上所述，红景天苷可能通过抑制细胞内Ca ~（2+）的过量内流和钙库的释放，对谷氨酸兴奋性毒性损伤的PC 12细胞起保护作用。
Salidroside (Sald), was extracted from Rhodiola rosea L, a traditional Chinese medicine which has been used for long time for anti-aging, anti-cancer and anti-oxidative stress etc. In present experiment, salidroside could protect the PC12 cell against injuries caused by exposure of PC12 cells to 2 mmol/L glutamate for 15 min followed by incubation with serum-free medium for 24 h, which resembled the excitotoxin in vivo system. Furthermore, saldroside could decrease the [Ca2+]i of PC12 cells in Mg2+ -free Hanks' solution and D-Hanks' solution but there was no effect on basal [Ca2+]i in Hanks' solution. The studies also indicated that salidroside inhibited the increases of [Ca2+]i induced by KCl and glutamate. In conclusion, salidroside may protect PC12 cell against glutamate excitotoxic damage through suppressing the excessive entry of Ca2+ and the release of the calcium stores.