The Role of Plant CCTα in Salt- and Osmotic-Stress Tolerance
The Role of Plant CCTα in Salt- and Osmotic-Stress Tolerance
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DOI：
10.1093/pcp/pcf120
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发表时间：
2002-09
影响因子：
4.9
通讯作者：
A. Yamada;M. Sekiguchi;T. Mimura;Y. Ozeki
中科院分区：
文献类型：
--
作者：
A. Yamada;M. Sekiguchi;T. Mimura;Y. Ozeki
；为寻找红树植物Bruguiera sexangula耐盐关键基因，以大肠杆菌为寄主进行了功能筛选。表达含有TCP-1 （CCT）同系物的细胞质伴侣蛋白的转化表现出增强的耐盐性。对大肠杆菌功能区域的分析显示，包含顶端结构域的218个氨基酸序列是渗透耐受性所必需的。此外，该结构域在体外表现出伴侣蛋白活性。因此，CCT促进了没有ATP或笼状结构的蛋白质的折叠，并可能在胁迫耐受性中发挥重要作用，至少在B. sexangula中是这样。
;To find key genes essential for salt tolerance in the mangrove plant, Bruguiera sexangula, functional screening was performed using Escherichia coli as the host organism. A transformant expressing a cytosolic chaperonincontaining TCP-1 (CCT) homologue displayed enhanced salt tolerance. Analysis in E. coli of the functional region revealed that a sequence of only 218 amino acids, containing the apical domain, is necessary for osmotolerance. Furthermore, this domain shows chaperone activity in vitro. Therefore, CCT facilitates the folding of proteins without ATP or the cage-like structure, and may play an important role in stress tolerance, at least in B. sexangula.