Engineering prevascularized composite cell sheet by light-induced cell sheet technology
Engineering prevascularized composite cell sheet by light-induced cell sheet technology
复制标题
利用光诱导细胞片技术工程化预血管化复合细胞片
DOI：
10.1039/c7ra05333a
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发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Wang Huiming
中科院分区：
文献类型：
--
作者：
Zhou Ying;Dong Lingqing;Liu Chao;Lin Yihan;Yu Mengfei;Ma Liang;Zhang Bin;Cheng Kui;Weng Wenjian;Wang Huiming
骨缺损的早期血管化被认为在骨再生过程中起关键作用。虽然间充质干细胞（MSC）与内皮细胞（EC）共培养在寻求实现血管化骨的策略中吸引了最多的关注，但在体外实现可以方便地翻译的预血管化构建体仍然是一个挑战。在此，我们提供了一种可转移的预血管化MSC-EC复合细胞片的工程策略，以促进骨组织工程中血管样网络的形成。我们将人间充质干细胞（hMSCs）和人脐静脉内皮细胞（HUVEC）在不同的光响应性TiO 2纳米点膜上共培养。探讨不同细胞比例的MSC-EC和培养液，以达到最佳的血管生成能力。预血管化的MSC-EC复合细胞片然后通过简单的光处理分离为完整和融合的细胞层，并显示出高活力和由间充质干细胞包围的3D网络形成。因此，这种光诱导细胞片技术实现了一种新的可转移的预血管化MSC-EC复合细胞片，并将对进一步设计骨组织工程的策略产生深远的影响。
Early vascularization in bone defects has been considered to play a critical role in the bone regeneration process. Although mesenchymal stem cells (MSCs) co-cultured with endothelial cells (ECs) have attracted the most attention in strategies that seek to achieve vascularized bone, it still remains a challenge to achieve a prevascularized construct in vitro that can be translated conveniently. Here, we provided a strategy of engineering a transferable prevascularized MSC–EC composite cell sheet to promote the vascular-like network formation for bone tissue engineering. We co-cultured human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on a distinct light-responsive TiO2 nanodots film. Various cell ratios of MSC–EC and culture mediums were explored to achieve the optimal angiogenesis capacity. The prevascularized MSC–EC composite cell sheet was then detached as an intact and confluent cell layer by a simple light treatment, and showed high viability and 3D network formation surrounded by mesenchymal stem cells. This light-induced cell sheet technology therefore realized a novel transferable prevascularized MSC–EC composite cell sheet, and will have a profound impact on further strategies for designing in-bone tissue engineering.