Biochemical Effects of High Hydrostatic Pressure on the Lysosome and Proteases Involved in It
Biochemical Effects of High Hydrostatic Pressure on the Lysosome and Proteases Involved in It
复制标题
高静水压对溶酶体及其相关蛋白酶的生化影响
DOI：
10.1271/bbb.56.1285
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. Hayashi
中科院分区：
文献类型：
--
作者：
T. Ohmori;T. Shigehisa;Siro Taji;R. Hayashi
对牛肝和分级溶酶体施加 101.3 至 506.6 MPa 的高压，以检查其对溶酶体和相关酶的影响。羧肽酶在405.3 MPa下完全失活。在506.6MPa加压后，酸性磷酸酶和组织蛋白酶B、D和H分别保持约60%、70%、30%和50%的活性。肝脏加压至 506.6 MPa 或更低会导致溶酶体酶的亚细胞分布发生变化。通过高于 202.7 MPa 的处理，酸性磷酸酶以及组织蛋白酶 B 和 H 从分级溶酶体中释放，组织蛋白酶 D 在 101.3 MPa 下处理。这些发现表明，101.3 至 202.7 MPa 的高压会导致溶酶体膜的破坏和溶酶体酶的泄漏。
High pressure at 101.3 to 506.6 MPa was applied to bovine liver and fractioned lysosomes to examine its effects on the lysosomes and the enzymes involved. Carboxypeptidase was completely inactivated at 405.3 MPa. The activities of about 60, 70, 30, and 50% of acid phosphatase and cathepsins B, D, and H, respectively, remained after pressurization at 506.6 MPa. Pressurization of the liver at 506.6 MPa or lower caused changes in the subcellular distribution of lysosomal enzymes. Acid phosphatase and cathepsins B and H were released from fractioned lysosome by treatment higher than 202.7 MPa and cathepsin D at 101.3 MPa. These findings indicate that high pressure at 101.3 to 202.7 MPa induces destruction of the lysosome membranes and leakage of the lysosomal enzymes.