Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress
Comparative physiological and proteomic analyses of mangrove plant Kandelia obovata under cold stress
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冷胁迫下红树植物秋茄的生理和蛋白质组学比较分析
DOI：
10.1007/s10646-021-02483-6
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发表时间：
2021-10-07
期刊：
影响因子：
2.7
通讯作者：
Sun, Cui-Ci
中科院分区：
文献类型：
--
作者：
Fei, Jiao;Wang, You-Shao;Sun, Cui-Ci
寒冷事件对红树林植物的生存和地理分布产生了广泛的影响。黄花菜（Kandelia obovata），作为一种真正的盐生植物和广泛分布的红树林物种，具有优异的耐寒性。研究了低温处理下倒卵黄叶片的生理特性和比较蛋白质组学。生理分析表明，黄颡鱼通过提高抗氧化剂水平、相关酶活性和渗透调节物质（脯氨酸）的活性来缓解其冷胁迫伤害。共检测到184个差异表达蛋白点，其中鉴定出129个（70.11%）。这些蛋白参与胁迫与防御、光合作用和光呼吸、信号转导、转录因子、蛋白质生物合成与降解、分子伴侣、ATP合成、三羧酸（TCA）循环和初级代谢等多种途径。蛋白质的翻译后修饰可能是一种普遍现象，在卵蛙的冷响应过程中起着关键作用。根据我们的宝贵工作，绘制了红树林植物在冷胁迫下的抗性或适应策略示意图。本研究为了解矮叶莲的耐冷机制提供了有价值的信息。
Cold events had broadly affected the survival and geographic distribution of mangrove plants. Kandelia obovata, has an excellent cold tolerance as a true halophyte and widespread mangrove species. In this study, physiological characters and comparative proteomics of leaves of K. obovata were performed under cold treatment. The physiological analysis showed that K. obovata could alleviate its cold-stress injuries through increasing the levels of antioxidants, the activities of related enzymes, as well as osmotic regulation substances (proline). It was detected 184 differentially expressed protein spots, and of 129 (70.11%) spots were identified. These proteins have been involved in several pathways such as the stress and defense, photosynthesis and photorespiration, signal transduction, transcription factors, protein biosynthesis and degradation, molecular chaperones, ATP synthesis, the tricarboxylic acid (TCA) cycle and primary metabolisms. The protein post-translational modification may be a common phenomenon and plays a key role in cold-response process in K. obovata. According to our precious work, a schematic diagram was drawn for the resistance or adaptation strategy of mangrove plants under cold stress. This study provided valuable information to understand the mechanism of cold tolerance of K. obovata.