The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model
The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model
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DOI：
10.1007/s10103-021-03354-6
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发表时间：
2021-07-02
影响因子：
2.1
通讯作者：
Park, Ilyong
中科院分区：
文献类型：
--
作者：
Lyu, Lele;Kim, Hyeongbeom;Park, Ilyong
本研究的目的是评估是否可以通过空间频域成像（SFDI）和激光散斑成像（LSI）在皮瓣小鼠模型中定量监测用于检测缺血性坏死的组织的血液灌注。在癌症研究所（ICR）小鼠上制作皮瓣。使用SFDI和LSI，估计以下参数：氧合血红蛋白（HbO（2））、脱氧血红蛋白（Hb）、总血红蛋白（THb）、组织氧饱和度（StO（2））和斑点血流指数（SFI）。组织学观察皮瓣组织的上皮厚度、胶原沉积、血管计数。应用斯皮尔曼等级相关分析法评价SFDI与组织学结果的相关性。结果显示，坏死组织组与非坏死组织组的血管数量和胶原面积百分比存在显著差异。尤其是坏死组织上皮完全丧失，失去正常结构。我们发现，SFDI/LSI参数之间的非坏死和坏死组织组有显着差异。尤其是术后第1天测量的所有SFDI和LSI参数在坏死组织和非坏死组织之间显示出显著差异。SFDI/LSI参数中血管数、胶原面积百分比与HbO（2）、StO（2）呈正相关。血管数目、胶原面积百分比与Hb呈负相关。通过同时应用SFDI和LSI，我们可以定量监测皮瓣的血液灌注和组织状况，有助于我们早期客观地发现缺血性坏死。
The aim of this study is to evaluate whether the blood perfusion to tissues for detecting ischemic necrosis can be quantitatively monitored by spatial frequency domain imaging (SFDI) and laser speckle imaging (LSI) in a skin flap mouse model. Skin flaps were made on Institute of Cancer Research (ICR) mice. Using SFDI and LSI, the following parameters were estimated: oxyhemoglobin (HbO(2)), deoxyhemoglobin (Hb), total hemoglobin (THb), tissue oxygen saturation (StO(2)), and speckle flow index (SFI). Histologically, epithelium thickness, collagen deposition, and blood vessel count of skin flap tissues were analyzed. Then, the correlation of SFDI and histological results was assessed by application of Spearman rank correlation method. As the result, the number of blood vessels and the percentage of collagen areas showed significant difference between the necrotic tissue group and the non-necrotic one. Especially, the necrotic tissue had a complete epithelial loss and loses its normal structure. We identified that SFDI/LSI parameters were significantly different between non-necrotic and necrotic tissue groups. Especially, all SFDI and LSI parameters measured on the 1st day after surgery showed significant difference between necrotic tissue and non-necrotic tissue. In addition, the number of blood vessel and percentage of collagen area were positively correlated with HbO(2) and StO(2) among SFDI/LSI parameters. Meanwhile, the number of blood vessel and percentage of collagen area showed the negative correlation with Hb. By applying SFDI and LSI simultaneously to the skin flap, we could quantitatively monitor the blood perfusion and the tissue condition which can help us to detect ischemic necrosis objectively in early stage.