Protective effects of nicergoline against neuronal cell death induced by activated microglia and astrocytes
Protective effects of nicergoline against neuronal cell death induced by activated microglia and astrocytes
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DOI：
10.1016/j.brainres.2005.10.050
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发表时间：
2005-12-20
期刊：
影响因子：
2.9
通讯作者：
Suzumura, A
中科院分区：
文献类型：
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作者：
Mizuno, T;Kuno, R;Suzumura, A
我们研究了尼麦角林在神经元-小胶质细胞或神经元-星形胶质细胞共培养物中的神经保护作用。尼麦角林是一种麦角林衍生物，可显着抑制由脂多糖 (LPS) 和干扰素 (IFN)-γ 刺激的活化小胶质细胞或星形胶质细胞共培养诱导的神经元细胞死亡。为了阐明尼麦角林发挥神经保护作用的机制，我们检查了尼麦角林治疗后激活的小胶质细胞和星形胶质细胞中炎症介质和神经营养因子的产生。在用 LPS 和 IFN-γ 刺激的小胶质细胞中，尼麦角林以剂量依赖性方式抑制超氧阴离子、白细胞介素 (IL)-1 β、IL-6 和肿瘤坏死因子 (TNF)-α 的产生。在星形胶质细胞中，尼麦角林还抑制促炎细胞因子的产生并增强脑源性神经营养因子（BDNF）。因此，尼麦角林介导的神经保护作用主要是由于神经胶质细胞抑制炎症介质和上调神经营养因子所致。 (c) 2005 Elsevier B.V. 保留所有权利。
We examined the neuroprotective role of nicergoline in neuron-microglia or neuron-astrocytes co-cultures. Nicergoline, an ergoline derivative, significantly suppressed the neuronal cell death induced by co-culture with activated microglia or astrocytes stimulated with lipopolysaccharide (LPS) and interferon (IFN)-gamma. To elucidate the mechanism by which nicergoline exerts a neuroprotective effect, we examined the production of inflammatory mediators and neurotrophic factors in activated microglia and astrocytes following nicergoline treatment. In microglia, stimulated with LPS and IFN-gamma, nicergoline suppressed the production of superoxide anions, interleukin (IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha in a dose-dependent manner. In astrocytes, nicergoline also suppressed the production of proinflammatory cytokines and enhanced brain-derived neurotrophic factor (BDNF). Thus, nicergoline-mediated neuroprotection resulted primarily from the inhibition of inflammatory mediators and the upregulation of neurotrophic factors by glial cells. (c) 2005 Elsevier B.V. All rights reserved.