Malagasy bats shelter a considerable genetic diversity of pathogenic Leptospira suggesting notable host-specificity patterns
Malagasy bats shelter a considerable genetic diversity of pathogenic Leptospira suggesting notable host-specificity patterns
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DOI：
10.1093/femsec/fiw037
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发表时间：
2016-04-01
影响因子：
4.2
通讯作者：
Tortosa, Pablo
中科院分区：
文献类型：
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作者：
Gomard, Yann;Dietrich, Muriel;Tortosa, Pablo
致病性钩端螺旋体是钩端螺旋体病的病原体，钩端螺旋体病是一种全球关注的疾病，主要影响热带地区。尽管这种人畜共患病对人类健康的重要性，但对宿主水库中形成细菌群落的进化和生态驱动因素的研究仍然很少。在这里，我们描述了钩端螺旋体群落主办的马达加斯加蝙蝠，主要是地方性物种组成，以表征主机-病原体协会和调查他们的进化历史。我们筛选了947个蝙蝠（代表31种，18属和7个家庭）钩端螺旋体感染，随后使用三种不同的细菌位点基因型阳性样本。分子生物学鉴定表明，这些钩端螺旋体具有明显的多样性，包括几个不同的谱系，主要属于Leptospira borgpetersenii和L。kirschneri。探索最可能的宿主-病原体进化情景表明，细菌遗传多样性是与生态学和宿主进化历史相关的事件的组合。重要的是，根据本文提供的数据集，我们发现的显着的主机特异性，以及缺乏细菌遗传多样性的地理结构，表明在给定的网站钩端螺旋体社区取决于共同出现的蝙蝠物种组合。这种严格的主机特异性的钩体病的流行病学的影响进行了讨论。
Pathogenic Leptospira are the causative agents of leptospirosis, a disease of global concern with major impact in tropical regions. Despite the importance of this zoonosis for human health, the evolutionary and ecological drivers shaping bacterial communities in host reservoirs remain poorly investigated. Here, we describe Leptospira communities hosted by Malagasy bats, composed of mostly endemic species, in order to characterize host-pathogen associations and investigate their evolutionary histories. We screened 947 individual bats (representing 31 species, 18 genera and seven families) for Leptospira infection and subsequently genotyped positive samples using three different bacterial loci. Molecular identification showed that these Leptospira are notably diverse and include several distinct lineages mostly belonging to Leptospira borgpetersenii and L. kirschneri. The exploration of the most probable host-pathogen evolutionary scenarios suggests that bacterial genetic diversity results from a combination of events related to the ecology and the evolutionary history of their hosts. Importantly, based on the data set presented herein, the notable host-specificity we have uncovered, together with a lack of geographical structuration of bacterial genetic diversity, indicates that the Leptospira community at a given site depends on the co-occurring bat species assemblage. The implications of such tight host-specificity on the epidemiology of leptospirosis are discussed.