SYNERGISTIC INDUCTION OF MESODERM BY FGF AND TGF-BETA AND THE IDENTIFICATION OF AN MESSENGER-RNA CODING FOR FGF IN THE EARLY XENOPUS EMBRYO
SYNERGISTIC INDUCTION OF MESODERM BY FGF AND TGF-BETA AND THE IDENTIFICATION OF AN MESSENGER-RNA CODING FOR FGF IN THE EARLY XENOPUS EMBRYO
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DOI：
10.1016/0092-8674(87)90110-3
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发表时间：
1987-12-04
期刊：
影响因子：
64.5
通讯作者：
KIRSCHNER, M
中科院分区：
文献类型：
--
作者：
KIMELMAN, D;KIRSCHNER, M
脊椎动物早期发育的主要模式事件是中胚层的形成及其随后的神经管的诱导。经典的实验表明，植物性区域向动物半球发出信号，使其从形成外胚层的途径转向形成中胚层，如肌肉。在此，我们表明牛碱性FGF具有有限的诱导肌肉肌动蛋白表达的能力，其可以被提高到在胚胎中由另一种哺乳动物生长因子TGF-β正常诱导的水平， 其本身不会诱导肌动蛋白表达。我们发现，非洲爪蟾胚胎含有一种mRNA编码的蛋白质高度同源的碱性成纤维细胞生长因子。这些结果连同与TGF-β具有强同源性的母体mRNA的鉴定，提示与FGF和TGF-β密切相关的分子。是脊椎动物发育中中胚层的天然诱导物。
The primary patterning event in early vertebrate development is the formation of the mesoderm and its subsequent induction of the neural tube. Classic experiments suggest that the vegetal region signals the animal hemisphere to diverge from the pathway of forming ectoderm to form mesoderm such as muscle. Here we show that bovine basic FGF has a limited capacity to induce muscle actin expression can be raised to levels normally induced in the embryo by another mammalian growth factor, TGF-.beta., which by itself will not induce actin expression. We show that the Xenopus embryo contains an mRNA encoding a protein highly homologous to basic FGF. These results together with the identification of a maternal mRNA with strong homology to TGF-.beta., suggest that molecules closely related to FGF and TGF-.beta. are the natural inducers of mesoderm in vertebrate development.