Sonic activation of molecularly-targeted nanoparticles accelerates transmembrane lipid delivery to cancer cells through contact-mediated mechanisms: Implications for enhanced local drug delivery
Sonic activation of molecularly-targeted nanoparticles accelerates transmembrane lipid delivery to cancer cells through contact-mediated mechanisms: Implications for enhanced local drug delivery
复制标题
DOI：
10.1016/j.ultrasmedbio.2005.07.022
复制
发表时间：
2005-12-01
影响因子：
2.9
通讯作者：
Wickline, SA
中科院分区：
文献类型：
--
作者：
Crowder, KC;Hughes, MS;Wickline, SA
液体全氟碳纳米颗粒通过与特定的细胞表面标志物结合，作为敏感和特定的靶向对比剂和药物输送载体。我们假设，在诊断功率水平上应用声能可以通过刺激纳米颗粒的脂层和靶细胞的质膜之间增加相互作用来促进纳米颗粒相关的药物传递。用常规超声成像系统对靶向C32黑色素瘤细胞的α(V)β(3)整合素的纳米颗粒施加超声(机械指数=1.9)，没有产生不良反应。在5分钟内，脂类从纳米粒输送到细胞质的能力显著增强。我们还演示了这一效应的潜在物理机制的操作，即对纳米颗粒的声辐射力，这可能有助于增强脂质输送。因此，我们建议，通过非破坏性机制，利用传统的超声成像设备可以快速、安全地将脂类物质(例如药物)从靶向纳米颗粒直接输送到细胞质中。(电子邮件：saw@wuPhy.wustl.edu)(C)2005年世界医学和生物学超声联合会。
Liquid perfluorocarbon nanoparticles serve as sensitive and specific targeted contrast and drug delivery vehicles by binding to specific cell surface markers. We hypothesized that application of acoustic energy at diagnostic power levels could promote nanoparticle-associated drug delivery by stimulating increased interaction between the nanoparticle's lipid layer and the targeted cell's plasma membrane. Ultrasound (mechanical index = 1.9) applied with a conventional ultrasound imaging system to nanoparticles targeted to alpha(v)beta(3)-integrins on C32 melanoma cancer cells in vitro produced no untoward effects. Within 5 min, lipid delivery from nanoparticles into cell cytoplasm was dramatically augmented. We also demonstrate the operation of a potential physical mechanism for this effect, the acoustic radiation force on the nanoparticles, which may contribute to the enhanced lipid delivery. Accordingly, we propose that local delivery of lipophilic substances (e.g., drugs) from targeted nanoparticles directly into cell cytoplasm can be augmented rapidly and safely with conventional ultrasound imaging devices through nondestructive mechanisms. (E-mail: saw@wuphys.wustl.edu) (c) 2005 World Federation for Ultrasound in Medicine & Biology.