Effects of Recombinant Human Bone Morphogenetic Protein-2 Dose and Ceramic Composition on New Bone Formation and Space Maintenance in a Canine Mandibular Ridge Saddle Defect Model.
Effects of Recombinant Human Bone Morphogenetic Protein-2 Dose and Ceramic Composition on New Bone Formation and Space Maintenance in a Canine Mandibular Ridge Saddle Defect Model.
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DOI：
10.1089/ten.tea.2015.0355
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发表时间：
2016-02
影响因子：
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通讯作者：
A. D. Talley;Kerem N. Kalpakci;Daniel A. Shimko;Katarzyna J. Zienkiewicz;D. Cochran;S. Guelcher
中科院分区：
文献类型：
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作者：
A. D. Talley;Kerem N. Kalpakci;Daniel A. Shimko;Katarzyna J. Zienkiewicz;D. Cochran;S. Guelcher
下颌骨缺损的治疗是一个重大的临床挑战。保持下颌骨嵴的高度和宽度对于种植体的植入和正常牙列的恢复至关重要。虽然使用保护膜的引导骨再生是维持牙槽嵴解剖轮廓和促进新骨形成的有效策略，但已报告了并发症，包括伤口失败、血清肿和导致感染的移植物暴露。在这项研究中，我们研究了可注射的低粘度（LV）聚氨酯/陶瓷复合材料增强100 μg/mL（低）或400 μg/mL（高）重组人骨形态发生蛋白-2（rhBMP-2）作为空间维持骨移植在犬下颌骨嵴鞍状缺损模型。LV移植物作为反应性糊剂注射，其在5-10分钟内凝固以形成体积模量超过IMPa的固体多孔复合材料。我们假设抗压左心室移植物可以促进新骨形成，并在不使用保护膜的情况下保持下颌骨嵴的解剖轮廓。在犬中可吸收胶原海绵载体推荐的rhBMP-2剂量（400 μg/mL）下，LV移植物保持了宿主下颌骨嵴的宽度和高度，并支持新骨形成，而在次优剂量（100 μg/mL）下，嵴的解剖轮廓没有保持。这些发现表明，体积模量超过1 MPa且rhBMP-2剂量与胶原海绵载体推荐剂量相当的抗压骨移植物支持新骨形成，并在无保护膜的下颌骨嵴缺损中保持嵴高度和宽度。
Treatment of mandibular osseous defects is a significant clinical challenge. Maintenance of the height and width of the mandibular ridge is essential for placement of dental implants and restoration of normal dentition. While guided bone regeneration using protective membranes is an effective strategy for maintaining the anatomic contour of the ridge and promoting new bone formation, complications have been reported, including wound failure, seroma, and graft exposure leading to infection. In this study, we investigated injectable low-viscosity (LV) polyurethane/ceramic composites augmented with 100 μg/mL (low) or 400 μg/mL (high) recombinant human bone morphogenetic protein-2 (rhBMP-2) as space-maintaining bone grafts in a canine mandibular ridge saddle defect model. LV grafts were injected as a reactive paste that set in 5-10 min to form a solid porous composite with bulk modulus exceeding 1 MPa. We hypothesized that compression-resistant LV grafts would enhance new bone formation and maintain the anatomic contour of the mandibular ridge without the use of protective membranes. At the rhBMP-2 dose recommended for the absorbable collagen sponge carrier in dogs (400 μg/mL), LV grafts maintained the width and height of the host mandibular ridge and supported new bone formation, while at suboptimal (100 μg/mL) doses, the anatomic contour of the ridge was not maintained. These findings indicate that compression-resistant bone grafts with bulk moduli exceeding 1 MPa and rhBMP-2 doses comparable to that recommended for the collagen sponge carrier support new bone formation and maintain ridge height and width in mandibular ridge defects without protective membranes.