Cocaine-induced locomotor activity is increased by prior handling in adolescent but not adult female rats.
Cocaine-induced locomotor activity is increased by prior handling in adolescent but not adult female rats.
复制标题
可卡因诱导的运动活动通过对青少年而非成年雌性大鼠的预先处理而增加。
DOI：
10.1016/j.physbeh.2005.08.026
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发表时间：
2005
影响因子：
2.9
通讯作者：
Kirstein,CherylL
中科院分区：
文献类型：
--
作者：
Maldonado,AntonietteM;Kirstein,CherylL
青春期是一个过渡时期，与压力水平增加和开始吸毒的倾向增加有关。在这个过渡时期，神经元的发育仍在进行，其中包括青春期多巴胺系统的持续发育。在本研究中，研究了前试处理对雌性青春期大鼠和年轻成年大鼠在呈现新环境时可卡因诱导的运动活动的影响。出生后第41-44天和第56-59只动物在菌落室处理（b.i.d） 3分钟。在PND 45或PND 60时，将动物从菌落室中取出，称重，并给予急性注射可卡因或生理盐水，并将其置于新环境中，记录其行为30分钟。在服用可卡因之前进行处理的青春期女性（PND 45），其可卡因诱导的活性水平相对于与其年龄匹配的未处理组和处理过的成年组都有所提高。相比之下，在未被处理的动物中，年轻成年动物（PND 60）在试验期间的几个时间点表现出药物引起的运动升高。与未被处理的成年动物相比，未被处理的青春期动物表现出先前描述的“反应迟钝”的行为特征。本实验结果表明，青春期动物可能对基本的实验室操作更敏感，如测试前处理，在使用青春期动物进行行为测试时必须注意。处理似乎是一个敏感的操纵在阐明不同年龄的可卡因诱导的行为激活的差异。
Adolescence is a period of transition that is associated with increased levels of stress and a heightened propensity to initiate drug use. Neuronal development is still occurring during this transitional period, which includes the continued development of the dopamine system during the adolescent period. In the present study, the effects of pre-test handling on cocaine-induced locomotor activity were investigated among female adolescent and young adult rats upon presentation to a novel environment. On postnatal days (PND) 41–44 and 56–59 animals were handled (b.i.d.) in the colony room for 3 min. On PND 45 or PND 60, animals were removed from the colony room, weighed, and administered an acute injection of either cocaine or saline and presented to a novel environment where behavior was recorded for 30 min. Adolescent females (PND 45) that were handled prior to cocaine administration demonstrated elevated levels of cocaine-induced activity relative to their age-matched non-handled counterparts and also to their handled-adult counterparts. In contrast, among non-handled animals, young adults (PND 60) exhibited elevated drug-induced locomotion at several time points during the trial. Non-handled adolescent animals demonstrated the previously described “hyporesponsive” behavioral profile relative to their non-handled adult counterparts. The results from the present experiment indicate that adolescent animals may be more sensitive to basic laboratory manipulations such as pre-test handling, and care must be taken when utilizing adolescent animals in behavioral testing. Handling appears to be a sensitive manipulation in elucidating differences in cocaine-induced behavioral activation between ages.