Trapping red blood cells in living animals using optical tweezers
Trapping red blood cells in living animals using optical tweezers
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使用光镊捕获活体动物中的红细胞
DOI：
10.1038/ncomms2786
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发表时间：
2013-04-01
影响因子：
16.6
通讯作者：
Li, Yin-Mei
中科院分区：
文献类型：
--
作者：
Zhong, Min-Cheng;Wei, Xun-Bin;Li, Yin-Mei
非侵入性成像技术的最新发展使活细胞中潜在的细胞过程的分子事件得以可视化。虽然可以很容易地在细胞水平上操纵微观物体，但通过操纵体内细胞很可能获得额外的生理洞察力，这到目前为止还没有实现。在这里，我们使用红外线光学镊子来捕获和操纵活着的小鼠皮下毛细血管内的红细胞。我们实现了一种非接触式的微操作，从而清除了堵塞的微血管。此外，我们还估算了毛细管中的光陷刚度。我们的工作扩大了光钳在动物活细胞动力学研究中的应用。
The recent development of non-invasive imaging techniques has enabled the visualization of molecular events underlying cellular processes in live cells. Although microscopic objects can be readily manipulated at the cellular level, additional physiological insight is likely to be gained by manipulation of cellsin vivo, which has not been achieved so far. Here we use infrared optical tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice. We realize a non-contact micro-operation that results in the clearing of a blocked microvessel. Furthermore, we estimate the optical trap stiffness in the capillary. Our work expands the application of optical tweezers to the study of live cell dynamics in animals.