Virus-like particles in Eimeria tenella are associated with multiple RNA segments
Virus-like particles in Eimeria tenella are associated with multiple RNA segments
复制标题
柔嫩艾美耳球虫中的病毒样颗粒与多个 RNA 片段相关
DOI：
10.1016/j.exppara.2010.12.005
复制
发表时间：
2011-03-01
影响因子：
2.1
通讯作者：
Zhang, Xichen
中科院分区：
文献类型：
--
作者：
Han, Qianzhong;Li, Jianhua;Zhang, Xichen
长春产柔弱艾美耳球虫孢子卵囊总核酸含有3个染色体外双链RNA片段（dsRNAs），大小分别为1.4、2.4和3.6 kb。这些rna在高盐浓度（0.3 M NaCl）下对RNase A的酶切具有抗性。对含有这些核酸种的柔毛绦虫孢子卵囊粗提物进行了RNA依赖性RNA聚合酶（RDRP）活性检测。经蔗糖密度梯度离心纯化后的病毒样颗粒（vlp）在电镜下直径约为38 nm。根据原生动物病毒通常采用的命名法，我们将该分离物命名为tenella E. virus (ETV)，这是首次从tenella E.分离到的病毒。(C) 2010爱思唯尔公司版权所有。
Total nucleic acids from sporulated oocysts of Eimeria tenella isolated from Changchun in China were found to contain three extrachromosomal double-stranded RNA segments (dsRNAs) of 1.4, 2.4 and 3.6 kb in sizes. These RNAs were resistant to RNase A digestion under high salt concentration (0.3 M NaCl). RNA-dependent RNA polymerase (RDRP) activity was detected in crude extracts of E. tenella sporulated oocysts containing these nucleic acid species. Virus-like particles (VLPs) were shown to have a diameter of approximately 38 nm under Electron Microscopy (EM) after purification by sucrose density gradient centrifugation. In keeping with the nomenclature generally adopted for protozoan viruses, we have named this isolate as E. tenella virus (ETV) which is the first virus isolated from E. tenella. (C) 2010 Elsevier Inc. All rights reserved.