Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients.
Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients.
复制标题
在胶体二氧化硅-聚乙烯吡咯烷酮梯度中分离各种血细胞。
DOI：
10.1016/0014-4827(68)90454-0
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发表时间：
1968
影响因子：
3.7
通讯作者：
Kerstin Lindahl
中科院分区：
文献类型：
--
作者：
Håkan Pertoft;O. Bäck;Kerstin Lindahl
描述了分离人血细胞成分的新方法。通过在胶体二氧化硅和聚乙烯吡咯烷酮的密度梯度中离心，可以将它们分离成红细胞、多形核白细胞、单核白细胞和血小板。该方法操作简单，产率和分离度均高于现有方法。在生理pH和渗透压的培养基中进行分级。通过荧光素二乙酸酯蓄积试验、台盼蓝排除试验和植物血凝素刺激后淋巴细胞组分中的胸苷掺入测定，分级分离后细胞是完整的。在所描述的技术中，一次可以分离1至200 ml的血液。或者，不同的细胞群可以在不同密度的二氧化硅溶液之间的界面处（在密度垫上）成带。后一种技术特别适用于处理大量血液。
New methods are described for the separation of the cellular components of human blood. They can be separated into erythrocytes, polymorpho-nuclear leucocytes, mononuclear leucocytes and platelets by centrifugation in density gradients of colloidal silica and polyvinylpyrrolidone. The procedures are simple and the yields and resolution achieved higher than in methods currently available. The fractionations are made in media of physiological pH and osmolality. The cells are intact after the fractionation as measured by the fluorescein diacetate accumulation test, the trypan blue exclusion test and thymidine incorporation in the lymphocyte fraction after phyto-hemagglutinin stimulation. In the techniques described, between 1 and 200 ml of blood can be fractionated at a time. Alternatively, the different cell populations may be banded at the interface between silica solutions of different densities (on density cushions). The latter technique is especially suitable for working up larger quantities of blood.