Mechanism of mutagenesis by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG). Methylation of nucleic acids by N‐trideuteriomethyl‐N′‐nitro‐N‐nitrosoguanidine (D3‐MNNG) in the presence of cysteine and in cells of Escherichia coli
Mechanism of mutagenesis by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG). Methylation of nucleic acids by N‐trideuteriomethyl‐N′‐nitro‐N‐nitrosoguanidine (D3‐MNNG) in the presence of cysteine and in cells of Escherichia coli
复制标题
N-甲基-N-硝基-N-亚硝基胍（MNNG）在半胱氨酸和存在下，N-三氘代甲基-N-硝基-N-亚硝基胍（D3-MNNG）对核酸进行甲基化的机制。在大肠杆菌细胞中
DOI：
10.1016/0014-5793(71)80544-6
复制
发表时间：
1971
期刊：
影响因子：
3.5
通讯作者：
R. Sussmuth
中科院分区：
文献类型：
--
作者：
F. Lingens;R. Haerlin;R. Sussmuth
MNNG的诱变作用被认为部分是由于核酸的甲基化。我们以前的结果可以排除DS-MNNG在体外pH 6.0时形成重氮甲烷作为DNA甲基化的中间产物。由于硫基化合物提高了核酸甲基化的速度[2]，我们在体外研究了半胱氨酸存在下Da-MNNG甲基化DNA的机制。此外，我们还研究了DS-MNNG在大肠杆菌B中体内甲基化核酸的机制。
The mutagenic effect of MNNG is considered to be in part due to methylation of nucleic acids. The formation of diazomethane as an intermediate of methylation of DNA by Ds-MNNG in vitro at pH 6.0 can be excluded by our former results [1]. As the rate of methylation of nucleic acids is enhanced by sulthydryl compounds [2], we studied the mechanism of methylation of DNA by Da-MNNG in vitro in the presence of cysteine. Furthermore, we studied the mechanism of methylation of nucleic acids by Ds-MNNG in vivo in Escherichia coli B.