DjERas plays an important role in planarian regeneration and homeostasis
DjERas plays an important role in planarian regeneration and homeostasis
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DjERas 在涡虫再生和稳态中发挥重要作用
DOI：
10.1016/j.bbrc.2019.04.118
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发表时间：
2019
影响因子：
3.1
通讯作者：
Zhang Shoutao
中科院分区：
文献类型：
--
作者：
Guo Qi;Ni Jiajia;Zhang Fangfang;Guo Yanan;Zhang Yizhe;Fang Huimin;Tian Qingnan;Zhang Shoutao
细胞更新的机制包括细胞增殖和细胞分化。Planarians拥有惊人的再生能力，并在一生中进行细胞更新。利用RNAi技术在三角洲三角洲中克隆到一个ERas同源基因。敲低DjERas导致再生和稳态缺陷。此外，我们还发现，在DjERas RNAi真涡虫中，新生细胞和晚期子代标记基因的表达降低。我们的研究表明，下调DjERas通过保守的信号通路抑制干细胞的增殖和分化，导致涡虫不能再生和维持稳态。我们的研究结果表明，DjERas在细胞周转过程中起着至关重要的作用。
The mechanisms of cell turnover including cell proliferation and cell differentiation were complex. Planarians possess amazing regeneration ability and undergo cell turnover throughout life. We identified a homologous gene of ERas by RNAi in Dugesia japonica. Knocking-down DjERas resulted in regeneration and homeostasis defects. Furthermore, we found that the expression of neoblasts and late progeny marker gene decreased in DjERas RNAi planarians. Our studies indicated that down-regulation of DjERas inhibited the proliferation and differentiation of stem cells through the conserved signaling pathway, resulted in the inability of the planarian to regenerate and maintain homeostasis. Our results suggest that DjERas plays a crucial role in the process of cell turnover.