Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa
Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa
复制标题
携带白菜基因组成分的甘蓝型油菜亚基因组间杂种优势的遗传解析
DOI：
10.1007/s10681-011-0533-8
复制
发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Meng, Jinling
中科院分区：
文献类型：
--
作者：
Fu, Donghui;Qian, Wei;Meng, Jinling
尽管在“天然”驯化的甘蓝型油菜（油菜籽，AACC）和一种新类型的油菜（其中油菜籽（AA）的亚基因组组分已经渗入）之间观察到了强的种子生产亚基因组间杂种优势，但是这种亚基因组间杂种优势的分子遗传机制尚不清楚。本研究以B为母本，通过杂交获得了一个新型油菜重组自交系群体。napus和B.以及来自与B的亲本系回交的群体。以甘蓝型油菜为材料，对产量和9个产量相关性状的单位点数量性状位点（QTL）和互作QTL对进行了鉴定。在检测到的单位点QTL和互作QTL对中，有一半以上与B导入所诱导的新等位基因有关。拉帕。等位基因直接来自B。rapa A基因组在亚基因组间杂种优势中起次要作用。由B产生的两种新等位基因的等位基因和非等位基因相互作用。rapa渐渗和直接来自B的等位基因。rapa A基因组对“天然”驯化油菜与新类型油菜之间的亚基因组间杂种优势有贡献，而B基因组对新类型油菜的亚基因组间杂种优势有贡献。拉帕已经渗入。鉴定了6个固定杂种优势位点，并讨论了它们的应用前景。
Although strong intersubgenomic heterosis for seed production has been observed between "natural'' domesticated Brassica napus (rapeseed, AACC) and a new type of rapeseed into which subgenomic components of Brassica rapa (AA) have been introgressed, the molecular genetic mechanism of this intersubgenomic heterosis is not understood. In this study, a recombinant inbred line population of new type rapeseed derived from a cross between B. napus and B. rapa, together with a population from a backcross with the parental line of B. napus, was used to identify single-locus quantitative trait locus (QTL) and interacting QTL pairs for yield and nine yield-related traits. More than half of single-locus QTLs and interacting QTL pairs detected were involved with the novel alleles induced by the introgression of B. rapa. The alleles directly from B. rapa A genome played a secondary role in contributing to intersubgenomic heterosis. Allelic and nonallelic interactions of both novel alleles generated by B. rapa introgression and the alleles directly from B. rapa A genome contributed to the intersubgenomic heterosis between "natural'' domesticated rapeseed and new type rapeseed into which B. rapa had been introgressed. Six loci for fixed heterosis were identified and their possible applications are also discussed.