Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency
Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency
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DOI：
10.1007/s11627-000-0007-5
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发表时间：
2000-01-01
影响因子：
2.6
通讯作者：
Wang, K
中科院分区：
文献类型：
--
作者：
Frame, BR;Zhang, HY;Wang, K
在这里，我们提出了一种常规而高效的方案，用于全年生产可育的转基因玉米植株。利用PDS 1000/He生物枪转化玉米Hi II未成熟合子胚II型愈伤组织，并在Bialaphos上进行筛选。为了改进转化方案，考察了金粒大小和愈伤组织形态对转化效率的影响。将金粒大小从1.0微米或0.6微米降至1.0微米或0.6微米，可显著提高II型愈伤组织中抗Bialaphos无性系的回收率。前胚性愈伤组织、早期胚性愈伤组织和晚期胚性愈伤组织的平均转化效率差异不显著。总结了II型愈伤组织的转化率、再生率和生育率，并与温室和田间来源的未成熟合子胚胎进行了比较。
Here we present a routine and efficient protocol for year-round production of fertile transgenic maize plants. Type II callus derived from maize Hi II immature zygotic embryos was transformed using the PDS 1000/He biolistic gun and selected on bialaphos. In an effort to improve the transformation protocol, the effects of gold particle size and callus morphology on transformation efficiency were investigated. Reducing gold particle size from 1.0 mu m or 0.6 mu m resulted in a significant increase in the rate of recovery of bialaphos-resistant clones from Type II callus. The average transformation efficiency of pre-embryogenic, early embryogenic and late embryogenic callus did not vary significantly. Rates of transformation, regeneration and fertility achieved for Type II callus are summarized and compared to those achieved for greenhouse- and field-derived immature zygotic embryos.