Tenascin-C is a useful marker for disease activity in myocarditis
Tenascin-C is a useful marker for disease activity in myocarditis
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DOI：
10.1002/path.1131
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发表时间：
2002-07-01
影响因子：
7.3
通讯作者：
Yoshida, T
中科院分区：
文献类型：
--
作者：
Imanaka-Yoshida, K;Hiroe, M;Yoshida, T
Tenascin-C （TNC）是一种细胞外基质蛋白，在胚胎发生或癌症侵袭过程中出现在组织重塑的活性位点。在正常的心脏中，TNC仅存在于发育的早期阶段，但在病理状态下会再次出现。本研究探讨TNC在评估心肌炎疾病活动性方面的诊断价值。在肌球蛋白诱导的自身免疫性心肌炎小鼠模型中检测TNC的表达。通过逆转录-聚合酶链反应、western blotting分析其数量、定位和产生细胞的顺序变化。免疫组化和原位杂交，并与组织学图比较。TNC的表达在心肌炎的早期就出现了上调。免疫染色在细胞浸润和肌细胞溶解变得明显之前可检测到，在活动期细胞浸润和坏死继续存在，在烧伤组织中随着愈合而消失。炎症灶坏死或退行性心肌细胞周围均可见TNC免疫染色，其表达水平与炎症活动的组织学证据相关。间质成纤维细胞是TNC的主要来源，表达含有不同剪接位点的大同种异构体。这些数据表明TNC是评估心肌炎疾病活动性的有用标记。版权所有:John Wiley Sons, Ltd。
Tenascin-C (TNC) is an extracellular matrix protein which appears at active sites of tissue remodelling during embryogenesis or cancer invasion. In normal heart, TNC is only present during the early stages of development but reappears in pathological states. This study examined the diagnostic value of TNC for assessing disease activity of myocarditis. Expression of TNC was examined in myosin-induced autoimmune myocarditis mouse models. Sequential changes in amount, localization and the producing cells were analysed by reverse transcriptase-polymerase chain reaction, western blotting. immunohistochemistry and in situ hybridization and compared with the histological picture. The expression of TNC was upregulated at a very early stage of myocarditis. Immunostaining was detectable before cell infiltration and myocytolysis became histologically apparent, remained during the active stage while cell infiltration and necrosis continued, and disappeared in sear tissue with healing. TNC immunostaining was always observed at the periphery of necrotic or degenerating cardiomyocytes in foci of inflammation, the expression level correlating with histological evidence of inflammatory activity. Interstitial fibroblasts were the major source of TNC, expressing the large isoform containing alternative splicing sites. These data demonstrate that TNC is a useful marker for evaluation of disease activity in myocarditis. Copyright (C) 2002 John Wiley Sons, Ltd.