T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis
T-cadherin inhibits invasion and migration of endometrial stromal cells in endometriosis
复制标题
T-钙粘蛋白抑制子宫内膜异位症中子宫内膜基质细胞的侵袭和迁移。
DOI：
10.1093/humrep/dez252
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发表时间：
2020-01-01
影响因子：
6.1
通讯作者：
Lash, Gendie E.
中科院分区：
文献类型：
--
作者：
Lu, Qinsheng;Huang, Yanqing;Lash, Gendie E.
研究问题：T-cadherin在子宫内膜异位症中的表达水平如何？T-cadherin是否在调节子宫内膜间质细胞的侵袭和迁移中发挥作用？与在位内膜相比，T-cadherin在异位内膜中的表达减少，T-cadherin的过度表达抑制了子宫内膜间质细胞的侵袭和迁移。子宫内膜异位症是一种涉及细胞侵袭和迁移的疾病。T-cadherin可以抑制各种癌细胞的细胞侵袭、迁移和增殖，但其在子宫内膜异位症中的作用还没有被研究过。研究设计、大小、持续时间我们探讨了40例子宫内膜异位症患者和24例非子宫内膜异位症患者中T-cadherin的表达状态。我们还分离了子宫内膜间质细胞，以研究T-cadherin过表达的侵袭、迁移和信号通路调节。参与者/材料，地点，方法患者在广州妇女儿童医疗中心招募，研究T-cadherin的表达水平。免疫组化染色和western blot检测T-cadherin的表达。采用H评分法评价T-cadherin的染色强度。分析T-cadherin表达水平（H评分）与子宫内膜异位症患者年龄、分期、病灶大小及粘连程度的相关性。从子宫内膜异位症患者和非子宫内膜异位症患者的子宫内膜基质细胞分离，并通过transwell检测T-钙粘蛋白过表达后的细胞侵袭和迁移。Western blot检测T-cadherin高表达细胞中波形蛋白的表达。在T-cadherin过度表达后，用Proteome Profiler Human Phospho-Kinase Array Kit检测信号通路蛋白的磷酸化谱。主要结果和机会的作用T-cadherin在对照患者的正常子宫内膜和子宫内膜异位症患者的在位子宫内膜中的表达没有差异，但在子宫内膜异位症患者的异位子宫内膜中显著降低，子宫内膜异位症患者在位内膜与正常子宫内膜比较（P
STUDY QUESTION What is the expression level of T-cadherin in endometriosis, and does T-cadherin play a role in regulating invasion and migration of endometrial stromal cells?SUMMARY ANSWER T-cadherin expression was reduced in ectopic endometriotic lesions compared to eutopic endometrium, and T-cadherin overexpression inhibited the invasion and migration of endometrial stromal cells.WHAT IS KNOWN ALREADY Endometriosis is a disease that involves active cell invasion and migration. T-cadherin can inhibit cell invasion, migration and proliferation in various cancer cells, but its role in endometriosis has not been investigated.STUDY DESIGN, SIZE, DURATION We explored the expression status of T-cadherin in 40 patients with and 24 without endometriosis. We also isolated endometrial stromal cells to study the invasion, migration and signaling pathway regulation of T-cadherin overexpression.PARTICIPANTS/MATERIALS, SETTING, METHODS Patients were recruited at the Guangzhou Women and Children's Medical Center to study the expression levels of T-cadherin. The expression of T-cadherin was detected by immunohistochemistry staining and western blot. H-score was used to evaluate the staining intensity of T-cadherin. The correlation between T-cadherin expression levels (H-score) and endometriosis patients' age, stage, lesion size and adhesion was analyzed. Endometrial stromal cells from patients with and without endometriosis were isolated, and cell invasion and migration were detected by transwell assays after T-cadherin overexpression. The expression of vimentin in T-cadherin-overexpressed cells was detected by western blot. After T-cadherin overexpression, the phosphorylation profile of signaling pathway proteins was detected with the Proteome Profiler Human Phospho-Kinase Array Kit.MAIN RESULTS AND THE ROLE OF CHANCE There was no difference in the expression of T-cadherin in the normal endometrium of control patients and the eutopic endometrium of endometriotic patients, but it was significantly decreased in the ectopic endometrium of endometriotic patients, compared with control endometrium and eutopic endometrium of endometriosis patients (P