Pregnancy-specific beta-1-glycoprotein (SP1) in cultured amniotic fluid cells.
Pregnancy-specific beta-1-glycoprotein (SP1) in cultured amniotic fluid cells.
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培养的羊水细胞中的妊娠特异性 β-1-糖蛋白 (SP1)。
DOI：
10.1210/jcem-51-6-1432
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
M. Seppälä
中科院分区：
文献类型：
--
作者：
M. Heikinheimo;T. Wahlström;P. Aula;I. Virtanen;M. Seppälä
利用RIA、免疫过氧化物酶和免疫荧光技术在羊水细胞培养中研究了妊娠特异性β -1糖蛋白（SP1）的合成。在所有11种超声波和26种培养基中的21种中均发现SP1。SP1免疫反应物质在免疫学上与母体血清SP1相似。免疫过氧化物酶和间接免疫荧光染色通过抗角蛋白抗体标记鉴定为羊膜上皮细胞的大细胞呈阳性。在培养中偶尔发现成纤维细胞样细胞，但它们不含有明显数量的SP1。这些发现的生理意义尚不清楚。
The synthesis of pregnancy-specific beta-1-glycoprotein (SP1) was studied in amniotic fluid cell cultures using RIA, immunoperoxidase, and immunofluorescence techniques. SP1 was found by RIA in all 11 sonicates and in 21 of 26 culture media. The SP1-immunoreactive material was immunologically similar to maternal serum SP1. Immunoperoxidase and indirect immunofluorescence staining were positive in large cells identified as epithelial amniotic cells by labeling with antikeratin antibodies. Fibroblast-like cells were occasionally found in cultures, but they did not contain demonstrable amounts of SP1. The physiological significance of the findings presented remains unclear.