Mechanical mapping of mammalian follicle development using Brillouin microscopy.
Mechanical mapping of mammalian follicle development using Brillouin microscopy.
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DOI：
10.1038/s42003-021-02662-5
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发表时间：
2021-09-27
影响因子：
5.9
通讯作者：
Prevedel R
中科院分区：
文献类型：
--
作者：
Chan CJ;Bevilacqua C;Prevedel R
在哺乳动物早期发育过程中，含有未成熟卵母细胞的卵泡的成熟是一个重要的生物学过程，因为功能性卵母细胞为成功繁殖提供了大量的遗传和细胞质材料。尽管最近的研究证明了机械应力在卵母细胞生长中的调节作用，但在体内和体外对卵巢力学特性的定量研究仍然缺乏。在这项工作中，我们使用布里渊显微镜（一种探测三维（3D）组织力学的非侵入性工具）量化了卵泡发育不同阶段的完整小鼠卵巢中的卵浆、卵泡和结缔组织的材料特性。我们发现卵巢皮质及其内部基质具有与细胞外基质沉积相关的独特物质特性，并且在卵泡成熟过程中出现了卵泡内机械室。我们的工作为研究机械在卵泡形态发生中的作用提供了另一种方法，并可能为未来理解生殖生物学中的机械转导铺平道路，对不孕症的诊断和治疗具有潜在的意义。Chan等人应用布里渊显微镜研究哺乳动物卵泡发育过程中组织材料特性的变化。聚焦于与折射率和质量密度无关的布里渊损失切线，他们发现哺乳动物卵泡发生的特征是由细胞分化和细胞外基质重塑驱动的组织微粘度的区域特异性变化。
In early mammalian development, the maturation of follicles containing the immature oocytes is an important biological process as the functional oocytes provide the bulk genetic and cytoplasmic materials for successful reproduction. Despite recent work demonstrating the regulatory role of mechanical stress in oocyte growth, quantitative studies of ovarian mechanical properties remain lacking both in vivo and ex vivo. In this work, we quantify the material properties of ooplasm, follicles and connective tissues in intact mouse ovaries at distinct stages of follicle development using Brillouin microscopy, a non-invasive tool to probe mechanics in three-dimensional (3D) tissues. We find that the ovarian cortex and its interior stroma have distinct material properties associated with extracellular matrix deposition, and that intra-follicular mechanical compartments emerge during follicle maturation. Our work provides an alternative approach to study the role of mechanics in follicle morphogenesis and might pave the way for future understanding of mechanotransduction in reproductive biology, with potential implications for infertility diagnosis and treatment. Chan et al. apply Brillouin microscopy to study changes in tissue material properties during mammalian follicle development. Focusing on the Brillouin loss tangent which is independent of the refractive index and mass density, they find that mammalian folliculogenesis is characterised by region-specific changes in tissue micro-viscosity driven by cell differentiation and extracellular matrix remodelling.