Expression of 17β and 3β-hydroxy steroid dehydrogenases and steroidogenic acute regulatory protein in non-luteinizing bovine granulosa cells in vitro
Expression of 17β and 3β-hydroxy steroid dehydrogenases and steroidogenic acute regulatory protein in non-luteinizing bovine granulosa cells in vitro
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DOI：
10.1016/j.mce.2004.05.010
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发表时间：
2004-08-31
影响因子：
4.1
通讯作者：
Price, CA
中科院分区：
文献类型：
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作者：
Sahmi, M;Nicola, ES;Price, CA
小卵泡的颗粒细胞在无血清培养液中分化，导致雌二醇分泌增加和编码细胞色素P450芳香化酶（P450 arom）的mRNA丰度增加。我们测试的假设，在体外分化也涉及增加表达的3 β-和17 β-羟基类固醇脱氢酶（HSD）的类固醇生成的急性调节蛋白（星星）的表达的情况下，已在体内观察到。颗粒细胞从小（
Granulosa cells of small follicles differentiate in vitro in serum-free medium, resulting in increased estradiol secretion and abundance of mRNA encoding cytochrome P450aromatase (P450arom). We tested the hypothesis that differentiation in vitro also involves increased expression of 3beta- and 17beta-hydroxysteroid dehydrogenases (HSD) in the absence of steroidogenic acute regulatory protein (StAR) expression, as has been observed in vivo. Granulosa cells from small (