Sneathiella aquimaris sp. nov., isolated from aquaculture seawater
Sneathiella aquimaris sp. nov., isolated from aquaculture seawater
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Snethiella aquimaris sp.
DOI：
10.1099/ijsem.0.004239
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发表时间：
2020
影响因子：
2.8
通讯作者：
Shao Zongze
中科院分区：
文献类型：
--
作者：
Li Guizhen;Lai Qiliang;Yan Peisheng;Gu Li;Shao Zongze
从南美白对虾（Penaeus vannamei）养殖水体中分离到一株新的海洋细菌，命名为216 LB-ZA 1 - 12 T。系统发育分析表明，菌株216 LB-ZA 1 - 12 T与Sneathiella glossodoripedis MKT 133 T的同源性最高（97.7%），其次为Sneathiella limimum-GH 1 - 24 T（97.0%）、Sneathiella chungangensisCAU 1294 T（96.6%）和Sneathiella chinensisLMG 23452 T（96.1%）。 216 LB-ZA 1 - 12 T株与4个近缘型菌株的平均核苷酸同源性在69.2-71.3%之间，DNA-DNA杂交估计值在16.7- 17.8%之间。 该细菌为革兰氏染色阴性，厌氧性，氧化酶和过氧化氢酶阳性，椭圆形至杆状，能动。在pH 7-9、1-15%的盐度和4-42 °C的温度下观察到生长。染色体DNA的G+C含量为48.50mol%。主要的呼吸醌被确定为Q-10。主要脂肪酸为特征8（C18：1ω 7 c/ω6c）和C16：0。 主要极性脂质为二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱和氨基磷脂。综合基因型和表型数据表明，菌株216 LB-ZA 1 - 12 T代表了Sneathiella属中的一个新种，其名称为Sneathiella aquimaciasp. nov.，模式菌株为216 LB-ZA 1 - 12 T（=MCCC 1A 14570 T =KCTC 72144 T）。
A novel marine bacterium, designated strain 216LB-ZA1-12T, was isolated from aPenaeus vannameiaquaculture seawater sample. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 216LB-ZA1-12Tbelonged to the genusSneathiella, with the highest sequence similarity toSneathiella glossodoripedisMKT133T(97.7 %), followed bySneathiella limimarisGH1-24T(97.0 %),Sneathiella chungangensisCAU 1294T(96.6 %) andSneathiella chinensisLMG 23452T(96.1 %). The average nucleotide identity and the DNA–DNA hybridization estimate values between strain 216LB-ZA1-12Tand four close type strains were between 69.2–71.3% and 16.7–17.8 %, respectively. The bacterium was Gram-stain-negative, facultatively anaerobic, oxidase and catalase positive, oval- to rod-shaped, and motile. Growth was observed at pH 7–9, salinities of 1–15% and temperatures of 4–42 °C. The G+C content of the chromosomal DNA was 48.50 mol%. The major respiratory quinone was determined to be Q-10. The principal fatty acids were summed feature 8 (C18 : 1ω7c/ω6c) and C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and aminophospholipid. The combined genotypic and phenotypic data show that strain 216LB-ZA1-12Trepresents a novel species within the genusSneathiella, for which the nameSneathiella aquimarissp. nov. is proposed, with the type strain 216LB-ZA1-12T(=MCCC 1A14570T=KCTC 72144T).