A predictive multiscale model for simulating flow-induced platelet activation: Correlating in silico results with in vitro results.
A predictive multiscale model for simulating flow-induced platelet activation: Correlating in silico results with in vitro results.
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DOI：
10.1016/j.jbiomech.2021.110275
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发表时间：
2021-03-05
影响因子：
2.4
通讯作者：
Bluestein D
中科院分区：
文献类型：
--
作者：
Zhang P;Sheriff J;Einav S;Slepian MJ;Deng Y;Bluestein D
Flow-induced platelet activation prompts complex filopodial formation. Continuum methods fail to capture such molecular-scale mechanisms. A multiscale numerical model was developed to simulate this activation process, where a Dissipative Particle Dynamics (DPD) model of viscous blood flow is interfaced with a Coarse Grained Molecular Dynamics (CGMD) platelet model. Embedded in DPD blood flow, the macroscopic dynamic stresses are interactively transferred to the CGMD model, inducing intra-platelet associated events. The platelets activate by a biomechanical transductive linkage chain and dynamically change their shape in response. The models are fully coupled via a hybrid-potential interface and multiple time-stepping (MTS) schemes for handling the disparity between the spatiotemporal scales. Cumulative hemodynamic stresses that may lead to platelet activation are mapped on the surface membrane and simultaneously transmitted to the cytoplasm and cytoskeleton. Upon activation, the flowing platelets lose their quiescent discoid shape and evolve by forming filopodia. The model predictions were validated by a set of in vitro experiments, Platelets were exposed to various combinations of shear stresses and durations in our programmable hemodynamic shearing device (HSD). Their shape change was measured at multiple time points using scanning electron microscopy (SEM). The CGMD model parameters were fine-tuned by interrogating a parameter space established in these experiments. Segmentation of the SEM imaging streams was conducted by a deep machine learning system. This model can be further employed to simulate shear mediated platelet activation thrombosis initiation and to study the effects of modulating platelet properties to enhance their shear resistance via mechanotransduction pathways.
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影响因子：
64.8
作者：
EGELMAN, EH;FRANCIS, N;DEROSIER, DJ
通讯作者：
DEROSIER, DJ
影响因子：
3.4
作者：
Lee, Dooyoung;Fong, Karen P.;Hammer, Daniel A.
通讯作者：
Hammer, Daniel A.
DOI：
10.1083/jcb.118.6.1421
发表时间：
1992-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hartwig JH
通讯作者：
Hartwig JH
影响因子：
4.1
作者：
Gao, Chao;Zhang, Peng;Bluestein, Danny
通讯作者：
Bluestein, Danny
影响因子：
2.8
作者：
Gupta, Prachi;Zhang, Peng;Deng, Yuefan
通讯作者：
Deng, Yuefan