Miniaturized multiplex label-free electronic chip for rapid nucleic acid analysis based on carbon nanotube nanoelectrode arrays
Miniaturized multiplex label-free electronic chip for rapid nucleic acid analysis based on carbon nanotube nanoelectrode arrays
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DOI：
10.1373/clinchem.2004.036285
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发表时间：
2004-10-01
影响因子：
9.3
通讯作者：
Li, J
中科院分区：
文献类型：
--
作者：
Koehne, JE;Chen, H;Li, J
背景：降低成本和时间是临床诊断，特别是分子诊断的主要关注点。小型化技术已被认为是提供用于诊断的低成本微芯片的有前途的解决方案。随着纳米技术的最新进展，有可能通过在诊断设备中引入纳米级元件来进一步提高检测灵敏度并简化样品制备。微米和纳米技术与生物学的融合具有巨大的潜力，为开发低成本的一次性芯片，快速分子分析，可以进行简单的手持device.Approach：垂直排列的多壁碳纳米管（MWNTs）上制作预沉积的微电极垫和封装在SiO2陶瓷，只有最后暴露在表面形成镶嵌纳米电极阵列（NEA）。NEA用于收集与结合到探针分子的靶分子相关的电化学信号，探针分子共价连接到MWNTs的末端。一个3 × 3的微电极阵列，展示了微型化和多路复用能力。随机分布的MWNT NEA制造在每个微电极垫上。选择性官能化的MWNT端与特定的寡核苷酸探针和钝化的二氧化硅表面与乙二醇部分进行了讨论。Ru（bpy）（2+）-介体放大的鸟嘌呤氧化用于直接测量与靶分子相关的电化学信号。总结：所讨论的MWNT NEAs在核酸靶中的鸟嘌呤碱基的直接电化学检测中具有很高的灵敏度。用一个20 × 20 μ m的微电极板可以测量不到1000个目标核酸分子，这接近基于荧光的DNA微阵列技术中激光扫描仪的检测极限。MWNT NEAs可以很容易地与微电子电路和微流体集成，用于开发全自动系统，以最小的成本进行快速分子分析。(C)2004年美国临床化学协会。
Background: Reducing cost and time is the major concern in clinical diagnostics, particularly in molecular diagnostics. Miniaturization technologies have been recognized as promising solutions to provide low-cost microchips for diagnostics. With the recent advancement in nanotechnologies, it is possible to further improve detection sensitivity and simplify sample preparation by incorporating nanoscale elements in diagnostics devices. A fusion of micro- and nanotechnologies with biology has great potential for the development of low-cost disposable chips for rapid molecular analysis that can be carried out with simple handheld devices.Approach: Vertically aligned multiwalled carbon nanotubes (MWNTs) are fabricated on predeposited microelectrode pads and encapsulated in SiO2 dielectrics with only the very end exposed at the surface to form an inlaid nanoelectrode array (NEA). The NEA is used to collect the electrochemical signal associated with the target molecules binding to the probe molecules, which are covalently attached to the end of the MWNTs.Content. A 3 X 3 microelectrode array is presented to demonstrate the miniaturization and multiplexing capability. A randomly distributed MWNT NEA is fabricated on each microelectrode pad. Selective functionalization of the MWNT end with a specific oligortucleotide probe and passivation of the SiO2 surface with ethylene glycol moieties are discussed. Ru(bpy)(2+)-mediator-amplified guanine oxidation is used to directly measure the electrochemical signal associated with target molecules.Summary: The discussed MWNT NEAs have ultrahigh sensitivity in direct electrochemical detection of guanine bases in the nucleic acid target. Fewer than similar to1000 target nucleic acid molecules can be measured with a single microelectrode pad of similar to20 X 20 mum(2), which approaches the detection limit of laser scanners in fluorescence-based DNA microarray techniques. MWNT NEAs can be easily integrated with microelectronic circuitry and microfluidics for development of a fully automated system for rapid molecular analysis with minimum cost. (C) 2004 American Association for Clinical Chemistry.