Prolonged duration of isoflurane anesthesia impairs spatial recognition memory through the activation of JNK1/2 in the hippocampus of mice
Prolonged duration of isoflurane anesthesia impairs spatial recognition memory through the activation of JNK1/2 in the hippocampus of mice
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DOI：
10.1097/wnr.0000000000000760
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发表时间：
2017-05-03
期刊：
影响因子：
1.7
通讯作者：
Chen, Ying
中科院分区：
文献类型：
--
作者：
Jiang, Shan;Miao, Bei;Chen, Ying
术后认知功能障碍是手术和麻醉的常见并发症，其潜在机制尚不清楚。我们的目的是研究不同持续时间的异氟醚麻醉对小鼠空间识别记忆和海马 JNK1/2 激活的影响。在本研究中，成年雄性小鼠用异氟烷麻醉不同持续时间（1.5% 异氟烷麻醉 1、2 和 4 小时）。麻醉后24小时在Y迷宫中使用自发交替和两次试验识别记忆来测定空间识别记忆。使用蛋白质印迹测试海马中 JNK1/2 的激活。与无麻醉组相比，用异氟醚治疗 4 小时的小鼠表现出显着减少的自发交替和降低的探查参数，但在用异氟醚治疗 1 或 2 小时的小鼠中没有观察到这一点。异氟烷麻醉1、2、4 h后10 min海马p-JNK1/2蛋白水平较不麻醉时显着升高。然而，仅异氟醚麻醉4小时仍会在麻醉后24小时增加JNK1/2和p-JNK1/2水平。我们得出的结论是，长时间的异氟醚麻醉维持了JNK1/2的激活，从而导致麻醉后24小时记忆障碍。版权所有 (C) 2017 Wolters Kluwer Health, Inc. 保留所有权利。
Postoperative cognitive dysfunction is a frequent complication with surgery and anesthesia, and the underlying mechanism is unclear. Our aim was to investigate the effect of different durations of isoflurane anesthesia on spatial recognition memory and activation of JNK1/2 in the hippocampus of mice. In the present study, adult male mice were anesthetized with isoflurane for different durations (1.5% isoflurane for 1, 2, and 4 h). Spatial recognition memory was determined using spontaneous alternation and two-trial recognition memory in Y-maze at 24 h after anesthesia. The activation of JNK1/2 in the hippocampus was tested using western blot. Mice treated with isoflurane for 4 h showed significantly decreased spontaneous alternations and decreased exploration parameters compared with the no anesthesia group, but this was not observed in mice treated with isoflurane for 1 or 2 h. The protein levels of p-JNK1/2 in the hippocampus were significantly increased at 10 min after isoflurane anesthesia for 1, 2, and 4 h compared with no anesthesia. However, only isoflurane anesthesia for 4 h still increased JNK1/2 and p-JNK1/2 levels at 24 h after anesthesia. We concluded that prolonged duration of isoflurane anesthesia maintained the activation of JNK1/2, which led to memory impairment at 24 h after anesthesia. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.