RATIO OF TEICHOIC ACID AND PEPTIDOGLYCAN IN CELL WALLS OF BACILLUS SUBTILIS FOLLOWING SPORE GERMINATION AND DURING VEGETATIVE GROWTH
RATIO OF TEICHOIC ACID AND PEPTIDOGLYCAN IN CELL WALLS OF BACILLUS SUBTILIS FOLLOWING SPORE GERMINATION AND DURING VEGETATIVE GROWTH
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DOI：
10.1128/jb.96.2.421-427.1968
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发表时间：
1968-01-01
影响因子：
3.2
通讯作者：
ENSIGN, JC
中科院分区：
文献类型：
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作者：
BOYLEN, CW;ENSIGN, JC
从B的细胞中分离细胞壁。subtlis菌株马尔堡在孢子同步生长过程中，在随后的2次同步细胞分裂过程中，以及在指数生长和早期稳定生长过程中的不同时间。测定每种细胞壁制备物中磷壁酸和肽聚糖组分的量。肽聚糖由己糖胺、丙氨酸、二氨基庚二酸和谷氨酸组成。在生长的每个阶段，细胞壁中这些物质的比例相对恒定。磷壁酸由甘油、磷酸盐、葡萄糖和酯连接的丙氨酸组成。除了葡萄糖和酯连接的丙氨酸，这些组件的比例是相对恒定的整个生长周期。随着细胞老化，磷壁酸的葡萄糖含量略有增加。酯连接的丙氨酸的量和生长阶段之间没有相关性。磷壁酸（基于磷酸盐含量）与肽聚糖（基于二氨基庚二酸含量）的比率在整个生长周期中保持在几乎恒定的水平。这两种细胞壁聚合物是在孢子生长过程中和整个营养生长周期中协同合成的。
Cell walls were isolated from cells of B. subtllis strain Marburg during synchronous outgrowth of spores, during the 2 synchronous cell divisions which followed and at various times during exponential and early stationary growth. The amounts of teichoic acid and peptidoglycan components were determined in each cell wall preparation. The peptidoglycan is composed of hexo-samine, alanine, diaminopimelic acid and glutamic acid. The ratio of these was relatively constant in the cell walls at each stage of growth. The teichoic acid is composed of glycerol, phosphate, glucose, and ester-linked alanine. With the exception of glucose and ester-linked alanine, the ratios of these components were relatively constant throughout the growth cycle. There was a slight increase in the glucose content of the teichoic acid as the cells aged. There was no correlation between the amount of ester-linked alanine and the stage of growth. The ratio of teichoic acid (based upon phosphate content) to peptidoglycan (based upon diaminopimelic acid content) remained at nearly a constant level throughout the growth cycle. The conclusion is presented that these 2 cell wall polymers are co-ordinately synthesized during spore outgrowth and throughout the vegetative growth cycle.