Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.
Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.
复制标题
使用淋巴结切片对间质 T 细胞迁移进行实时成像。
DOI:
10.1007/978-1-4939-7762-8_4
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发表时间:
2018
期刊:
影响因子:
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通讯作者:
Katakai T.
中科院分区:
文献类型:
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作者:
浦島晶子;名川文清;名川文清;名川文清;名川文清;Katakai T.
Live imaging using various microscopic technologies is an indispensable tool for investigating the dynamic nature of immune cells. One of the most powerful techniques is the two-photon laser-scanning microscopy (TP-LSM), which has various advantages in observing deep tissues in vivo. Interstitial T cell migration in the lymph node (LN) is a phenomenon intensively examined using TP-LSM in the field of immunology. Intravital and explant methods have been standards for imaging T cell behaviors in the LN, though there are several limitations. Live imaging of LN slices, an LN explant sliced by a vibratome to expose tissue parenchyma, could provide an alternative approach with technical advantages for an in-depth analysis of interstitial T cell migration in vivo.
DOI:
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发表时间:
2010
期刊:
影响因子:
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作者:
Kim;M.;Katagiri K.;et. al.
通讯作者:
et. al.