Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.

Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices.
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使用淋巴结切片对间质 T 细胞迁移进行实时成像。

DOI:
10.1007/978-1-4939-7762-8_4
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发表时间:
2018
期刊:
Methods Mol Biol.
影响因子:
--
通讯作者:
Katakai T.
Katakai T.
中科院分区:
--
文献类型:
--
作者:
浦島晶子;名川文清;名川文清;名川文清;名川文清;Katakai T.

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使用各种显微技术的实时成像是研究免疫细胞动态性质不可或缺的工具。其中最强大的技术是双光子激光扫描显微镜(TP-LSM),它在观察体内深部组织方面具有各种优势。间质T细胞在淋巴结(LN)的迁移是一种现象,在免疫学领域使用TP-LSM进行了深入研究。尽管存在一些局限性,活体和外植体方法已成为LN中T细胞行为成像的标准方法。LN切片的实时成像,通过振动器切片LN外植体以暴露组织实质,可以为深入分析体内间质T细胞迁移提供一种具有技术优势的替代方法。
Live imaging using various microscopic technologies is an indispensable tool for investigating the dynamic nature of immune cells. One of the most powerful techniques is the two-photon laser-scanning microscopy (TP-LSM), which has various advantages in observing deep tissues in vivo. Interstitial T cell migration in the lymph node (LN) is a phenomenon intensively examined using TP-LSM in the field of immunology. Intravital and explant methods have been standards for imaging T cell behaviors in the LN, though there are several limitations. Live imaging of LN slices, an LN explant sliced by a vibratome to expose tissue parenchyma, could provide an alternative approach with technical advantages for an in-depth analysis of interstitial T cell migration in vivo.
Mst1 控制淋巴结内淋巴细胞的运输和运动
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者:
Kim;M.;Katagiri K.;et. al.
通讯作者: et. al.