Ultrastructure and Sequential Development of Infectious Pancreatic Necrosis Virus
Ultrastructure and Sequential Development of Infectious Pancreatic Necrosis Virus
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传染性胰腺坏死病毒的超微结构及后续研究
DOI：
10.1128/jvi.3.1.52-58.1969
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发表时间：
1969
影响因子：
5.4
通讯作者：
M. Gravell
中科院分区：
文献类型：
--
作者：
L. Moss;M. Gravell
通过电子显微镜研究了鳟鱼病原体传染性胰腺坏死（IPN）病毒的形态和顺序发育。感染后 6 小时，在 24°C 下孵育的受感染细胞的细胞质中即可看到成熟的病毒颗粒。这些病毒颗粒的轮廓呈六边形，直径约为 55 nm。一般在感染后8至10小时内，偶尔会观察到不同大小的病毒晶体。尽管病毒复制似乎并不局限于特定的细胞质位点，但有时会看到成熟的病毒粒子与外径约为 45 nm 的未识别管状结构相关。病毒的阴性染色显示无包膜的二十面体，直径约 65 nm，可能有 92 个壳粒。与之前报道的 IPN 病毒具有类似小核糖核酸病毒形态的通讯相反，我们发现它在形态上类似于呼肠孤病毒组的成员。
The morphology and sequential development of infectious pancreatic necrosis (IPN) virus, a pathogen of trouts, were studied by electron microscopy. Mature virions were seen in the cytoplasm of infected cells incubated at 24 C as early as 6 hr after infection. These virions were hexagonal in profile and approximately 55 nm in diameter. Generally between 8 to 10 hr after infection, virus crystals of various sizes were occasionally observed. Although virus replication did not appear to be confined to a particular cytoplasmic locus, mature virions were sometimes seen in association with unidentified tubular structures approximately 45 nm in outside diameter. Negative stains of virus revealed unenveloped icosahedra approximately 65 nm in diameter with probably 92 capsomeres. Contrary to a previous communication which reported IPN virus to have picornavirus-like morphology, we found it to morphologically resemble members of the reovirus group.