Toll receptor response to white spot syndrome virus challenge in giant freshwater prawns (Macrobrachium rosenbergii)
Toll receptor response to white spot syndrome virus challenge in giant freshwater prawns (Macrobrachium rosenbergii)
复制标题
Toll受体对巨型淡水虾（罗氏沼虾）白斑综合症病毒攻击的反应
DOI：
10.1016/j.fsi.2016.08.017
复制
发表时间：
2016-10-01
影响因子：
4.7
通讯作者：
Ren, Qian
中科院分区：
文献类型：
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作者：
Feng, Jinling;Zhao, Lingling;Ren, Qian
Toll 受体是模式识别受体的进化古老家族，在无脊椎动物先天免疫反应中发挥着至关重要的作用。在这项研究中，我们从巨型淡水虾（罗氏沼虾）中鉴定出了 Toll 受体 (MrToll)。 MrToll的cDNA全长为4257 bp，编码1367个氨基酸的推定蛋白。 MrToll 包含 17 个 LRR 结构域、一个跨膜结构域和一个 TIR 结构域。系统发育分析表明，MrToll 与果蝇 Toll7 和其他节肢动物 Toll 属于同一类。 MrToll的转录本主要分布在心脏、肝胰腺、鳃、胃和肠。在血细胞和淋巴器官中可以检测到低水平的 MrToll 表达。在白斑综合症病毒（WSSV）攻击期间，从24小时到48小时，鳃中MrToll的表达逐渐上调至最高水平。鳃中Cm基因Cru3和Cru7的表达量相对低于Cru2和Cru4。在 WSSV 攻击过程中，MrToll RNA 干扰后，鳃中 Cru3 和 Cru7 的表达水平受到抑制。在病毒攻击期间，鳃中的抗脂多糖因子（ALF）基因 ALF2、ALF3、ALF4 和 ALF5 也受到 MrToll 的调节。这些发现表明，MrToll 可能有助于罗氏沼虾针对 WSSV 的先天免疫防御。 (C) 2016 Elsevier Ltd. 保留所有权利。
Toll receptors are evolutionary ancient families of pattern recognition receptors with crucial roles in invertebrate innate immune response. In this study, we identified a Toll receptor (MrToll) from giant freshwater prawns (Macrobrachium rosenbergii). The full-length cDNA of MrToll is 4257 bp, which encodes a putative protein of 1367 amino acids. MrToll contains 17 LRR domains, a transmembrane domain, and a TIR domain. Phylogenetic analysis showed that MrToll was grouped with Drosophila Toll7 and other arthropod Tolls. The transcripts of MrToll are mainly distributed in the heart, hepatopancreas, gills, stomach, and intestine. A low level of MrToll expression can be detected in hemocytes and the lymphoid organ. MrToll expression in gills was gradually upregulated to the highest level from 24 h to 48 h during the white spot syndrome virus (WSSV) challenge. The expression levels of the crustin (Cm) genes Cru3 and Cru7 in gills were relatively lower than those of Cru2 and Cru4. The expression levels of Cru3 and Cru7 were inhibited after the RNA interference of MrToll in gills during the WSSV challenge. The antilipopolysaccharide factor (ALF) genes ALF2, ALF3, ALF4, and ALF5 were also regulated by MrToll in gills during the virus challenge. These findings suggest that MrToll may contribute to the innate immune defense of M. rosenbergii against WSSV. (C) 2016 Elsevier Ltd. All rights reserved.