Effects of mechanical stress on cytokine production in mandible-derived osteoblasts.
Effects of mechanical stress on cytokine production in mandible-derived osteoblasts.
复制标题
DOI：
10.1111/j.1601-0825.2011.01832.x
复制
发表时间：
2011-10
期刊：
影响因子：
3.8
通讯作者：
Kenta Yamamoto;Toshiro Yamamoto;Hiroaki Ichioka;Yuki Akamatsu;F. Oseko;O. Mazda;Jiro Imanishi;N. Kanamura;Masakazu Kita
中科院分区：
文献类型：
--
作者：
Kenta Yamamoto;Toshiro Yamamoto;Hiroaki Ichioka;Yuki Akamatsu;F. Oseko;O. Mazda;Jiro Imanishi;N. Kanamura;Masakazu Kita
目的众所周知，机械应力是骨重塑调节的重要因素，下颌骨持续暴露于咬合力等机械应力源下。因此，在本研究中，我们使用原始的静水压装置研究了接近咬合力的机械应力（下颌骨来源的成骨细胞（MDOB）所暴露的机械应力）对细胞因子表达和产生的影响。材料和方法 通过实时 RT-PCR、ELISA 和蛋白质印迹法检测 MDOB 中的细胞因子水平。此外，还使用 ERK1/2、JNK 和 p-38 途径的丝裂原激活蛋白激酶抑制剂来鉴定信号转导途径。结果 静水压以幅度和时间依赖性方式增加 IL-6 和 TNF-α mRNA 的表达，并且还增强 IL-6 和 TNF-α 蛋白的产生。此外，静水压改变了 RANKL/OPG 比率，有利于 mRNA 和蛋白质水平的 RANKL。 p-38 途径的特异性抑制剂（而非 ERK1/2 和 JNK 途径的抑制剂）抑制静水压负荷诱导的 RANKL 产生的上调。结论 这些结果表明 MDOB 在响应机械应力的细胞因子产生中发挥作用，并且咬合力可能通过破骨细胞生成激活骨重塑来支持下颌骨稳态的维持。
OBJECTIVE Mechanical stress is known to be an important factor in the regulation of bone remodeling, and mandibular bone is continuously exposed to mechanical stressors such as occlusal force. Therefore, in this study, we investigated the effects of mechanical stress approaching occlusal force, to which mandible-derived osteoblasts (MDOB) are exposed, on cytokine expression and production using an original hydrostatic pressure apparatus. MATERIALS AND METHODS The levels of cytokine in MDOB were examined by real-time RT-PCR, ELISA, and western blotting. In addition, mitogen-activated protein kinase inhibitor for ERK1/2, JNK, and p-38 pathways was used to identify the signal transduction pathway. RESULTS Hydrostatic pressure increased the expression of IL-6 and TNF-α mRNA in a magnitude- and time-dependent manner and also enhanced IL-6 and TNF-α protein production. Furthermore, hydrostatic pressure changed the RANKL/OPG ratio in favor of RANKL for both mRNA and protein levels. Specific inhibitor of p-38 pathway but not that of the ERK1/2 and JNK pathways suppressed the up-regulation of RANKL production induced by hydrostatic pressure loading. CONCLUSION These results suggest that MDOB play a role in cytokine production in response to mechanical stress and that occlusal force may support the maintenance of mandible bone homeostasis by activating bone remodeling through osteoclastogenesis.