THE DIFFERENTIATION OF MYELOID LEUKAEMIA CELLS: NEW POSSIBILITIES FOR THERAPY
THE DIFFERENTIATION OF MYELOID LEUKAEMIA CELLS: NEW POSSIBILITIES FOR THERAPY
复制标题
粒细胞白血病细胞的分化：治疗的新可能性
DOI：
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发表时间：
1978
影响因子：
6.5
通讯作者：
L. Sachs
中科院分区：
文献类型：
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作者：
L. Sachs
The development of experimental systems for the culture and cloning of normal haematopoietic cells (Ginsburg & Sachs, 1963, 1965; Sachs, 1964, 1974a, b; Pluznik & Sachs, 1965, 1966; Ichikawa et al, 1966; Bradley & Metcalf, 1966; Paran et al , 1970), has made it possible to study the controls that regulate haematopoietic cell differentiation and the blocks that can occur in luekaemia. All the main types of mammalian haematopoietic cells can be cloned in culture (Ginsburg & Sachs, 1963; Pluznik & Sachs, 1965; Ichikawa et al , 1966; Bradley & Metcalf, 1966; Stephenson et a l , 1971; Metcalfet al, 1975; Gerassi & Sachs, 1976; Fibach et al , 1976; Sredni et a l , 1976). Normal myeloid precursors can be induced to differentiate to mature macrophages and granulocytes by the protein inducer (Pluznik & Sachs, 1965,1966; Ichikawa et a l , 1976) that we now call MGI (macrophage and granulocyte inducer) (Landau & Sachs, 1971; Sachs, 1974a, b). This inducer is secreted by various types of cells including fibroblasts and macrophages and can also be found in human serum (Mintz & Sachs, 1973b). Unless otherwise stated, all the experiments described in this paper have been carried out with cells from mice. MGI, which has also been referred to as mashran gm (Ichikawa et al , 1967), colony stimulating factor (Metcalf, 1969) or colony stimulating activity (Austin et al, 1971), is specific for the induction of macrophages and granulocytes. Purified MGI from fibroblasts has a molecular weight of about 68 ooo (Landau & Sachs, 1971; Guez & Sachs, 1973; Stanley & Heard, 1977), purified MGI from lungs a molecular weight of about 23 ooo (Burgess et al, 1977) and the lower molecular weight may be derived from the higher molecular weight MGI. Purified MGI can induce the formation of macrophages and granulocytes. There may, however, also be molecular forms that induce only macrophages or granulocytes and different co-factors (Landau & Sachs, 1971; Sachs, 1974b) that can influence the differentiation to one or the other cell type. Incubation of the precursor cells with MGI for different periods of time has indicated that MGI has to be present until the process of differentiation is completed (Pluznik & Sachs, 1966; Paran & Sachs, 1968). Normal myeloid precursors require MGI for cell viability, growth and differentiation. It remains to be determined whether these properties can all be induced by MGI molecules with the same chemical composition.
