Properties of hemagglutination by Prevotella melaninogenica
Properties of hemagglutination by Prevotella melaninogenica
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DOI：
10.1016/j.anaerobe.2005.02.004
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发表时间：
2005-10-01
期刊：
影响因子：
2.3
通讯作者：
Holbrook, WP
中科院分区：
文献类型：
--
作者：
Haraldsson, G;Meurman, JH;Holbrook, WP
虽然产黑色素普雷沃氏菌属于口腔微生物群，但有些菌株具有推定的毒力因子。例如，我们先前描述了从牙周病患者中分离的生黑色素原杆菌的菌毛血凝菌株。本研究的目的是比较产黑色素P. melaninogenica与其他革兰氏阴性厌氧菌血凝（HA）的某些化学和物理性质。HA的13个P.melaninogenica菌株被证明是相当弱的牙周主要病原体，牙龈卟啉单胞菌。剧烈振荡降低了振荡细胞的HA，但振荡上清液具有与非振荡细胞相同的血凝活性。黑色素原卟啉单胞菌上的血凝剂似乎是一种蛋白质，它可以从细胞中分离出来，并与红细胞上含乳糖、半乳糖和棉子糖的碳水化合物结合。黑色素原卟啉单胞菌的血凝性和非血凝性菌株对上皮细胞的粘附性没有显著差异。虽然黑色素原卟啉单胞菌能够凝集红细胞，但这种潜在的毒力因子比主要牙周病原体的毒力因子低得多。(c)2005爱思唯尔有限公司保留所有权利。
Although Prevotella melaninogenica belongs to the commensal oral microbiota, some strains possess putative virulence factors. For example, we have previously described fimbriated, hemagglutinating strains of P. melaninogenica, isolated from patients with periodontal disease. The aim of this investigation was to compare some chemical and physical properties of hemagglutination (HA) of P. melaninogenica with those of other pigmented gram-negative anaerobes. HA of 13 P. melaninogenica strains proved to be considerably weaker than that of the major periodontal pathogen, Porphyromonas gingivalis. Vigorous shaking reduced HA of shaken cells but the shaken supernatant had the same hemagglutinating activity as non-shaken cells. The hemagglutinating agent on P. melaninogenica seemed to be a protein, which can be separated from the cell and binds to lactose-, galactose-, and raffinose-containing carbohydrates on the erythrocytes. Adherence to epithelial cells did not differ significantly between the hemagglutinating and non-hemagglutinating strains of P. melaninogenica. Although P. melaninogenica is able to agglutinate erythrocytes, this potential virulence factor is of a considerably lower magnitude than that of major periodontal pathogens. (c) 2005 Elsevier Ltd. All rights reserved.