Impact of probiotics on colonic microflora in patients with colitis: A prospective double blind randomised crossover study
Impact of probiotics on colonic microflora in patients with colitis: A prospective double blind randomised crossover study
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DOI：
10.1016/j.ijsu.2013.08.019
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发表时间：
2013-01-01
影响因子：
15.3
通讯作者：
MacFie, John
中科院分区：
文献类型：
--
作者：
Ahmed, Jamil;Reddy, Bala S.;MacFie, John
背景与目的：这项研究的目的是调查结肠炎患者的结肠菌群谱，以及用合生元治疗一个月是否可以改变这种情况。方法：这是一项试验性研究，患者被随机分为两组，一组接受合生元制剂一个月，然后“交叉”接受安慰剂，另一组在第二个月先接受安慰剂，第二个月再接受合生元。分别在研究开始时、第一个月和第二个月末采集粪便样本。用末端限制性片段长度多态技术检测结肠微生物区系。结果：16例患者完成研究，其中8例为克罗恩结肠炎，8例为溃疡性结肠炎。他们的平均年龄为62岁(智商50-65岁)。在每个患者中平均鉴定出22个末端限制性片段(T-RF)。骰子聚类分析表明，每个患者都有独特的微生物组成，无论他们是否服用益生菌或安慰剂，在研究的不同时间点都没有显著变化。在粪便样本中发现了益生菌，但没有改变微生物区系的总体谱。在这项初步研究中，我们无法确定任何与结肠炎性质相关的特定特征。结论：本研究表明，克罗恩病或溃疡性结肠炎患者之间的结肠微生物区系没有差异，合生元给药没有改变细菌的光谱。(C)2013 Surgical Associates Ltd.由爱思唯尔有限公司出版。版权所有。
Background & aims: : The aim of this study was to investigate the spectrum of colonic microflora in patients with colitis and if this could be altered with one month's treatment with synbiotics.Methods: This was a pilot study in which patients were randomised to either receive a synbiotics preparation for a month and then "crossed over" to receive a placebo, or alternatively to receive the placebo first followed in the second month by synbiotic. Stool samples were collected on entry into the study and then at the end of first and second months respectively. Colonic microflora was measured by terminal restriction fragment length polymorphism technique. Quantitative PCR was used to determine the concentration of individual species.Results: Sixteen patients completed the study of whom 8 had Crohn's colitis and 8 had ulcerative colitis. Their median age was 62 (IQR 50-65) years. An average of 22 terminal restriction fragments (T-RF's) was identified in each patient. Dice cluster analysis showed that each patient had a unique microbial composition which did not change significantly at different time points in the study, irrespective of whether they had probiotics or the placebo. Probiotic organisms were identified in stool samples but did not alter overall spectrum of microflora. In this pilot study we were unable to identify any specific characteristics related to nature of colitis.Conclusions: This study suggests that there is no difference in colonic microflora between patients with Crohn's or Ulcerative colitis and that the spectrum of bacteria was not altered by synbiotic administration. (C) 2013 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.