Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.
Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.
复制标题
编码衣壳蛋白和 RNA 聚合酶的甲型肝炎病毒 cDNA 的序列分析。
DOI：
10.1073/pnas.82.7.2143
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发表时间：
1985
影响因子：
11.1
通讯作者：
S. Feinstone
中科院分区：
文献类型：
--
作者：
B. Baroudy;J. Ticehurst;Thomas A. Miele;Jacob V. Maizel;Robert H. Purcell;S. Feinstone
我们在这里报告了与甲型肝炎病毒(HAV)基因组两大区域相对应的核苷酸序列。这些序列包括3274个碱基，对应于基因组的5‘端，包括这种微小核糖核酸病毒推测的衣壳蛋白区域，以及1590个碱基，对应于基因组的3’端，终止于15个碱基的聚(A)区。这些序列揭示了甲型肝炎病毒具有微小核糖核酸病毒特有的基因组结构：从RNA的5‘端开始约750个碱基的开放阅读框和来自3’聚(A)区的终止密码子60个碱基。预测的这两个区域的氨基酸序列已经与之前为其他小核糖核酸病毒确定的类似区域进行了比较。有足够的同源性得出结论，甲型肝炎病毒5‘端编码衣壳蛋白，3’端编码RNA聚合酶。然而，没有发现甲型肝炎病毒的这些区域与脊髓灰质炎病毒、脑心肌炎病毒和口蹄疫病毒的类似区域密切相关。
We report here the nucleotide sequence corresponding to two large regions of the hepatitis A virus (HAV) genome. These comprise a sequence of 3274 bases corresponding to the 5' end of the genome, which includes the putative capsid protein region of this picornavirus, and 1590 bases corresponding to the 3' end of the genome, terminating in a 15-base poly(A) tract. These sequences revealed that HAV had the characteristic genomic organization of picornaviruses: an open reading frame beginning approximately 750 bases from the 5' end of the RNA and a termination codon 60 bases from the 3' poly(A) tract. The predicted amino acid sequences of both regions have been compared to analogous regions previously determined for other picornaviruses. There was sufficient homology to conclude that the 5' region of HAV codes for capsid proteins and that the 3' region codes for an RNA polymerase. However, these regions of HAV were not found to be closely related to analogous regions of poliovirus, encephalomyocarditis virus, and foot and mouth disease virus.