A Combination of Exercise and Amino Acid Mixture Supplementation Comprised of Arginine, Alanine and Phenylalanine Reduces Diet—induced Obesity in Mice
A Combination of Exercise and Amino Acid Mixture Supplementation Comprised of Arginine, Alanine and Phenylalanine Reduces Diet—induced Obesity in Mice
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运动与补充由精氨酸、丙氨酸和苯丙氨酸组成的氨基酸混合物的组合可减少饮食引起的小鼠肥胖
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发表时间：
2017
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通讯作者：
Keisuke Ueda
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作者：
Keisuke Ueda
在早期的报告中，我们开发了一种由精氨酸、丙氨酸和苯丙氨酸组成的新型氨基酸（AA）混合物（A-mix），并表明运动前摄入A-mix可以通过增加肾上腺素和胰高血糖素的分泌来增加运动期间（Ex）和Ex后的血液酮体和甘油水平。然而，尚不清楚如果摄入补充剂数周，这种由摄入 A-mix 和 Ex 组合引起的急性反应是否会持续。在此，我们探讨了 A-mix 与 Ex 对高脂饮食（HF）喂养的 C57BL╱6 小鼠肥胖发展的长期影响。我们比较了在跑步机上跑步 Ex 补充或不补充 A-mix（1 g╱kg╱天）的 5 周期间的效果。我们发现，HF+A-mix 可抑制 HF 引起的体重增加 19％，并发现接受 A-mix 补充剂的组肠系膜脂肪重量显着减少。此外，HF+A-mix组棕色脂肪组织（BAT）中过氧化物酶体增殖物激活受体γ共激活剂1-α（PGC1-α）水平显着升高。总而言之，这些结果表明，膳食 A-mix 补充剂与持续的 Ex 相结合，可以通过调节 BAT 中 PGC1-α 的水平来减轻饮食诱导的肥胖，而 BAT 参与线粒体的生物合成和功能。
In earlier reports, we developed a novel amino acid（AA）mixture comprised of arginine, alanine, and phenylalanine（A-mix）, and suggested that pre-exercise ingestion of A-mix increases levels of blood ketone bodies and glycerol both during exercise（Ex）and following a bout of Ex by increasing secretion of adrenalin and glucagon. However, it is unclear whether this acute response induced by ingestion of A-mix combined with Ex is sustained if the supplement is ingested for several weeks. Herein, we explored the long-term effects of A-mix with Ex on the development of obesity in C57BL╱6 mice that were fed a high-fat diet （HF）. We compared the effects over a 5-week period utilizing treadmill-running Ex with or without A-mix（1 g╱kg╱day）supplementation. We found that HF＋A-mix suppressed HF-induced bodyweight gain by 19％, and found that the weight of mesenteric fat was significantly reduced in the group that received the A-mix supplement. Moreover, levels of peroxisome proliferator-activated receptor gamma coactivator 1-alpha（PGC1-α）in brown adipose tissue（BAT）were significantly increased in the HF＋A-mix group. Taken together, these results suggest that dietary A-mix supplementation, in combination with continuous Ex, attenuates diet-induced obesity by modulating levels of PGC1-α in BAT, which is involved in mitochondrial biogenesis and function.