Practical procedure for estimation of corticosterone or hydrocortisone.
Practical procedure for estimation of corticosterone or hydrocortisone.
复制标题
评估皮质酮或氢化可的松的实用程序。
作者：
R. Silber;R. Busch;R. Oslapas
本文介绍了一种测定皮质酮或氢化可的松的简化荧光法。尽管有一定的特异性限制，它有实际的应用，估计这些类固醇的样品是小体积或低浓度，或在研究中，主要是为了证明增加或减少肾上腺皮质激素在血浆或肾上腺，而不需要绝对的特异性。对于大鼠、人、猴或豚鼠血浆，0.5 ml。足以进行分析。一个大鼠肾上腺或豚鼠肾上腺的一部分通常含有足够的类固醇进行一次测定。还描述了可应用于大鼠血浆以校正干扰荧光物质的程序。
A simplified fluorometric procedure for corticosterone or hydrocortisone has been described. In spite of certain specificity limitations, it has practical applications for the estimation of these steroids in samples that are small in volume or low in concentration, or in studies that are designed primarily to demonstrate increases or decreases in adrenocortical hormones in plasma or adrenals without requiring absolute specificity. For rat, human, monkey, or guinea pig plasma, 0.5 ml. suffices for an analysis. One rat adrenal or a fraction of a guinea pig adrenal usually contains enough steroid for a single determination. A procedure also has been described which can be applied to rat plasma to correct for interfering fluorescent material.