Increased levels of a TH2-type CC chemokine thymus and activation-regulated chemokine (TARC) in serum and induced sputum of asthmatics
Increased levels of a TH2-type CC chemokine thymus and activation-regulated chemokine (TARC) in serum and induced sputum of asthmatics
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DOI：
10.1034/j.1398-9995.2002.1s3256.x
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发表时间：
2002-02-01
期刊：
影响因子：
12.4
通讯作者：
Hirai, K
中科院分区：
文献类型：
--
作者：
Sekiya, T;Yamada, H;Hirai, K
背景：TH2细胞释放的细胞因子在哮喘发病机制中起重要作用。最近的研究表明，CC趋化因子受体(CCR)4在TH2细胞中优先表达。这些事实表明，两种CCR4特异性配体，即胸腺和活化调节趋化因子(TARC)和巨噬细胞衍生趋化因子(MDC)，可能通过TH2细胞募集到炎症部位参与了哮喘的发病。方法：采用高灵敏度的双抗体夹心ELISA法测定46例哮喘患者和26例健康人的血清和诱导痰中TARC的水平。结果：哮喘患者外周血嗜酸粒细胞趋化因子水平显著升高(P<；0.001)，而外周血嗜酸粒细胞数量无明显变化(P>0.05)。虽然我们的检测系统在大多数病例的痰中检测不到mDC，但痰中TARC显著升高(P=0.027)。结论：哮喘患者痰中TARC水平升高可能参与了哮喘的病理生理过程。
Background: Cytokines liberated by TH2 cells play crucial roles in the pathogenesis of bronchial asthma. Recent studies have demonstrated that CC chemokine receptor (CCR)4 is preferentially expressed by TH2 cells. These facts suggest possible involvement of two CCR4-specific ligands i.e., thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC), in the pathogenesis of bronchial asthma via recruitment of TH2 cells to inflammatory sites. We investigated the levels of TARC and MDC in the serum and induced sputum of asthmatics.Methods: The levels of TARC in the serum (46 asthmatics and 26 healthy subjects) and induced sputum (30 asthmatics and 6 healthy subjects) were measured using a highly sensitive ELISA system. The levels of eotaxin and MDC were also measured by ELISA.Results: TARC, but not MDC, was significantly increased in asthmatic sera (P < 0.001). Although MDC was undetectable in the sputum of most cases by our assay system, sputum TARC was significantly increased (P = 0.027).Conclusions: The elevated TARC levels in asthmatics might be involved in the pathophysiology of asthma.