Histamine elimination by a coupling reaction of fungal amine oxidase and bacterial aldehyde oxidase
Histamine elimination by a coupling reaction of fungal amine oxidase and bacterial aldehyde oxidase
复制标题
通过真菌胺氧化酶和细菌醛氧化酶的偶联反应消除组胺
DOI：
10.1093/bbb/zbac121
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Osao Adachi
中科院分区：
文献类型：
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作者：
Masakatsu Usui;Hikari Kubota;Mizuki Ishihara;Haruka Matsuki;Shinya Kawabe;Yoshimasa Sugiura;Naoya Kataoka,Kazunobu Matsushita;Yoshitaka Ano;Yoshihiko Akakabe;Roque A. Hours;Toshiharu Yakushi; Osao Adachi
鱼类和水产品中通过细菌氨基酸脱羧酶的作用积累的组胺（HIST）和其他生物胺不能通过加热或其他化学方法分解和消除。提出了一种通过乙酸菌的真菌胺氧化酶 (FAO) 和细菌醛氧化酶 (ALOX) 的偶联反应消除 HIST 的简单方法。作为模型反应，FAO 将苯甲胺氧化为苯甲醛，苯甲醛又被 ALOX 自发氧化为苯甲酸。很可能，在 HIST 消除过程中，FAO 与 ALOX 很好地结合，从 HIST 中生产咪唑 4-乙酸，表观收率为 100%。在反应混合物中未检测到咪唑4-乙醛。在不存在 ALOX 的情况下，由于反应混合物中存在许多不明物质，偶联反应不完全。所提出的偶联酶法可能非常有效地消除鱼类和水产品中的有毒胺。
Histamine (HIST) and other biogenic amines found in fish and fishery products accumulated by the action of bacterial amino acid decarboxylase cannot be decomposed and eliminated by heating or other chemical methods. A simple method for HIST elimination is proposed by a coupling reaction of the fungal amine oxidase (FAO) and bacterial aldehyde oxidase (ALOX) of acetic acid bacteria. As a model reaction, FAO oxidized benzylamine to benzaldehyde, which in turn was oxidized spontaneously to benzoic acid with ALOX. Likely, in HIST elimination, FAO coupled well with ALOX to produce imidazole 4-acetic acid from HIST with an apparent yield of 100%. Imidazole 4-acetaldehyde was not detected in the reaction mixture. In the absence of ALOX, the coupling reaction was incomplete given a number of unidentified substances in the reaction mixture. The proposed coupling enzymatic method may be highly effective to eliminate toxic amines from fish and fishery products.