DNA microarray experiments: Biological and technological aspects
DNA microarray experiments: Biological and technological aspects
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DOI：
10.1111/j.0006-341x.2002.00701.x
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发表时间：
2002-12-01
期刊：
影响因子：
1.9
通讯作者：
Carroll, RJ
中科院分区：
文献类型：
--
作者：
Nguyen, DV;Arpat, AB;Carroll, RJ
DNA微阵列技术，如cDNA和寡核苷酸微阵列，有望彻底改变生物学研究和进一步我们对生物过程的理解。由于微阵列实验产生的复杂性质和大量数据，生物学家寻求分析科学专家的合作，包括统计学家等。然而，微阵列实验的生物学和技术复杂性对分析专家来说并不容易理解。本综述的目的之一是为涉及微阵列实验的一些相关生物学和技术方面提供一个桥梁。虽然已经有大量关于该技术广泛应用的文献，但对该技术本身的基础研究以及了解过程变化的研究仍处于起步阶段。我们强调DNA阵列技术基础研究的重要性，以提高未来实验的可靠性。
DNA microarray technologies, such as cDNA and oligonucleotide microarrays, promise to revolutionize biological research and further our understanding of biological processes. Due to the complex nature and sheer amount of data produced from microarray experiments, biologists have sought the collaboration of experts in the analytical sciences, including statisticians, among others. However, the biological and technical intricacies of microarray experiments are not easily accessible to analytical experts. One aim for this review is to provide a bridge to some of the relevant biological and technical aspects involved in microarray experiments. While there is already a large literature on the broad applications of the technology, basic research on the technology itself and studies to understand process variation remain in their infancy. We emphasize the importance of basic research in DNA array technologies to improve the reliability of future experiments.