Novel insights into the fundamentals of palatal shelf elevation dynamics in normal mouse embryos
Novel insights into the fundamentals of palatal shelf elevation dynamics in normal mouse embryos
复制标题
对正常小鼠胚胎腭架抬高动力学基本原理的新见解
DOI：
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
A. Czirók
中科院分区：
文献类型：
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作者：
I. Saadi;Jeremy P. Goering;Michael Moedritzer;Marta Stetsiv;D. Isai;Brittany M Hufft;Zaid Umar;Madison K. Rickabaugh;P. Keselman;Munish Chauhan;William M. Brooks;Kenneth Fischer;A. Czirók
胚胎腭发育涉及腭架的双侧垂直生长-从上颌突起延伸-靠近舌头，直到胚胎日（E）13.5。在垂直生长之后，到E14.5，腭架升高并粘附在舌头上方。目前的模型表明，这一过程的海拔涉及一个复杂的垂直到水平的腭架重新定向。虽然早期的研究暗示这是一个快速的过程，但确切的时间尚未解决。为了了解腭架抬高的动力学，我们采用了一小时分辨率的限时妊娠和中期的磁共振成像。我们的数据表明，在几乎所有的C57 BL/6 J胚胎，腭架尚未升高E14.0。然而，6小时后，在E14.25，80%的胚胎的腭架已经完成了升高。有趣的是，所有E14.25胚胎与未升高的腭架（20%）是女性，这表明在女性胚胎的延迟。在FVB/NJ胚胎中，升高窗口开始较早（E13.875-E14.25），没有任何明显的性别差异。我们经常捕捉到一个中间阶段，单侧隆起的右或左腭架。不同时期的磁共振成像显示，腭架抬高开始于后部双侧隆起的形成。随着时间的推移，这些隆起向外侧和前侧发展。在升高过程中，我们观察到腭架舌侧区域的细胞增殖增加。在隆起，细胞的方向是急剧倾斜的舌头和肌动球蛋白的活性增加，这一起可能参与在水平方向上的凸起的投影。因此，我们的数据揭示了新的见解腭架高度的快速动态变化，为今后的正常和异常腭发育的研究奠定了基础。
Embryonic palate development involves bilateral vertical growth of palatal shelves – extensions from the maxillary processes – next to the tongue until embryonic day (E) 13.5. Following vertical growth, palatal shelves elevate and adhere above the tongue by E14.5. Current models indicate that this process of elevation involves a complex vertical to horizontal reorienting of the palatal shelves. While earlier studies have implied that this is a rapid process, the precise timing has not been resolved. To understand the dynamics of palatal shelf elevation, we employed time-restricted pregnancies with a one-hour resolution and magnetic resonance imaging of intermediate stages. Our data showed that in almost all C57BL/6J embryos, palatal shelves have not yet elevated by E14.0. However, six hours later at E14.25, palatal shelves have completed elevation in 80% of embryos. Interestingly, all E14.25 embryos with unelevated palatal shelves (20%) were female, suggesting a delay in female embryos. In FVB/NJ embryos, the elevation window started earlier (E13.875-E14.25) without any noticeable sex differences. We frequently captured an intermediate stage with unilateral elevation of either right or left palatal shelf. Magnetic resonance imaging of various stages showed that palatal shelf elevation began with the formation of bilateral bulges in the posterior. These bulges progressed laterally and anteriorly over time. During elevation, we observed increased cell proliferation in the lingual region of the palatal shelf. Within the bulge, cell orientation was acutely tilted towards the tongue and actomyosin activity was increased, which together may participate in the projection of the bulge in the horizontal direction. Thus, our data reveal novel insights into the rapid dynamic changes in palatal shelf elevation that lay the foundation for future studies of normal and abnormal palatogenesis.
影响因子：
9.8
作者：
Leslie, Elizabeth J.;Taub, Margaret A.;Murray, Jeffrey C.
通讯作者：
Murray, Jeffrey C.
影响因子：
9.8
作者：
Leslie, Elizabeth J.;Liu, Huan;Marazita, Mary L.
通讯作者：
Marazita, Mary L.
DOI：
10.1016/0002-9416(82)90211-1
发表时间：
1982
期刊：
American journal of orthodontics
影响因子：
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作者：
Slavkin,HC;Melnick,M
通讯作者：
Melnick,M