Melatonin and Peripheral Circuitries: Insights on Appetite and Metabolism in Danio Rerio
Melatonin and Peripheral Circuitries: Insights on Appetite and Metabolism in Danio Rerio
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DOI：
10.1089/zeb.2012.0844
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发表时间：
2013-09-01
期刊：
影响因子：
2
通讯作者：
Carnevali, Oliana
中科院分区：
文献类型：
--
作者：
Piccinetti, Chiara Carla;Migliarini, Beatrice;Carnevali, Oliana
褪黑激素是昼夜节律的神经内分泌传感器，能够同步生物体的生理活动。根据我们最近的研究结果，食欲调节褪黑激素在斑马鱼大脑中，本研究的目的是评估褪黑激素的外周电路调节食物摄入，生长和脂质代谢的作用。为此，在生理和分子水平上评估了通过水向成年斑马鱼施用两种褪黑激素剂量（100 nM和1 μ M）10天的效果。同时分析了控制能量稳态的主要信号。此外，褪黑激素剂量对肌肉代谢资源的影响进行了评估。所获得的结果表明，褪黑激素通过刺激参与食欲抑制的分子，例如肝脏和肠中的瘦素（LPT）和肝脏中的MC 4 R（黑皮质素系统受体）来减少食物摄入。此外，褪黑激素降低肝脏胰岛素样生长因子-I（IGF-I）基因表达，其参与生长过程和参与脂质代谢的其它信号，如增殖物激活受体（PPAR α、β和γ）和固醇调节元件结合蛋白（SREBP）。这些结果与肌肉中较低水平的脂质相关，如大分子池分析所证明的。在这项研究中获得的结果可能是一个更好地理解的分子机制作为食物摄入量控制的基础，反过来，可以是一个有用的工具，为医疗和水产养殖应用的极大兴趣。
Melatonin is a neuroendocrine transducer of circadian/circannual rhythms able to synchronize organism's physiological activity. On the basis of our recent findings on appetite regulation by melatonin in the zebrafish brain, the aim of this study was to evaluate melatonin's role in peripheral circuitries regulating food intake, growth, and lipid metabolism. For this purpose, the effect of two melatonin doses (100nM and 1 mu M) administered for 10 days, via water, to adult zebrafish was evaluated at both physiological and molecular levels. The major signals controlling energy homeostasis were analyzed together. Additionally, the effect of melatonin doses on muscle metabolic resources was evaluated. The results obtained indicate that melatonin reduces food intake by stimulating molecules involved in appetite inhibition, such as leptin (LPT), in the liver and intestine and MC4R, a melanocortin system receptor, in the liver. Moreover, melatonin decreases hepatic insulin-like growth factor-I (IGF-I) gene expression, involved in growth process and other signals involved in lipid metabolism such as proliferator-activated receptors (PPAR alpha, beta, and gamma) and sterol regulatory element-binding protein (SREBP). These results were correlated with lower levels of lipids in the muscles as evidenced by the macromolecular pools analyses. The findings obtained in this study could be of great interest for a better understanding of the molecular mechanisms as the basis of food intake control and, in turn, can be a useful tool for medical and aquaculture applications.