A SPLICING ENHANCER EXHIBITS BOTH CONSTITUTIVE AND REGULATED ACTIVITIES
A SPLICING ENHANCER EXHIBITS BOTH CONSTITUTIVE AND REGULATED ACTIVITIES
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DOI：
10.1101/gad.8.14.1703
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发表时间：
1994-07-15
影响因子：
10.5
通讯作者：
MANIATIS, T
中科院分区：
文献类型：
--
作者：
TIAN, M;MANIATIS, T
果蝇蛋白质Transformer（Tra）和Transformed（Tra 2）通过特异性结合位于雌性特异性3'剪接位点下游300个核苷酸（nt）的剪接增强子（dsx重复元件; dsxRE）来调节果蝇doublemex（dsx）前mRNA的性别特异性选择性剪接。在本文中，我们表明，dsxRE可以作为一个Tra和Tra 2-独立的剪接增强子在体外时，位于150个核苷酸的3'剪接位点。基于在Tra和Tra 2的存在或不存在下稳定结合到dsxRE的SR蛋白的相对水平，我们提出dsxRE的组成型剪接活性是由其与SR蛋白和可能的其他一般剪接因子的弱相互作用介导的。相比之下，Tra和Tra 2通过稳定这些蛋白质与dsxRE之间的相互作用，允许dsxRE在距离内含子一定距离处起作用。
The Drosophila proteins Transformer (Tra) and Transformed (Tra2) regulate the sex-specific alternative splicing of Drosophila doublesex (dsx) pre-mRNA by specifically binding to a splicing enhancer (dsx repeat element; dsxRE) located 300 nucleotides (nt) downstream from a female-specific 3' splice site. In this paper we show that the dsxRE can function as a Tra and Tra2-independent splicing enhancer in vitro when located within 150 nucleotides of the 3' splice site. Based on the relative levels of SR proteins that bind stably to the dsxRE in the presence or absence of Tra and Tra2, we propose that the constitutive splicing activity of the dsxRE is mediated by its weak interactions with SR proteins and possibly other general splicing factors. In contrast, Tra and Tra2 allow the dsxRE to function at a distance from the intron by stabilizing the interactions between these proteins and the dsxRE.