Aquaporin channels may modulate ventilator-induced lung injury
Aquaporin channels may modulate ventilator-induced lung injury
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DOI：
10.1016/s0034-5687(00)00193-6
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Quinn, DA
中科院分区：
文献类型：
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作者：
Hales, CA;Du, HK;Quinn, DA
成人呼吸窘迫综合征是一种具有功能性肺异质性的疾病，因此呼吸机输送的呼吸可能过度扩张非受累区域。在大鼠中，我们检查了呼吸机输送的潮气量（TV）呼吸7和20 ml/kg的肺水作为肺损伤的证据。我们研究了水通道蛋白在呼吸机诱导的肺损伤（VILI）中的作用，通过输注HgCl 2，其通过结合半胱氨酸抑制水通道蛋白。作为肺水证据的肺湿干重比（W/D）在对照组中为4.47 +/- 0.1 SEM，在以7和20 ml/kg通气的大鼠中分别为4.6 +/- 0.1和5.5 +/- 0.2（P < 0.05）。在以30 ml/kg通气的大鼠中，肺动脉压（PAP）从23 +/- 1上升到26 +/- 1 mmHg（P < 0.05，n = 7），心输出量从104 +/- 2下降到67 +/- 3 ml/min（P < 0.05）。左心室舒张末期压（n = 3）无变化。在TV 20大鼠中，白蛋白标记物伊文思蓝染料从对照组的37 +/- 11增加到97 +/- 41 mg/g湿肺（P < 0.05）。尾静脉缓慢输注HgCl 2对肺动脉压无明显影响，但能使通气量为20 ml/kg的大鼠W/D增加到6 ± 0.2（P < 0.05），而对7 ml/kg的大鼠则无明显影响。等摩尔半胱氨酸：输注可防止HgCl 2使W/D增加至高于TV 20 ml/kg时所见的水平。因此，20 ml/kg TV通气产生了富含蛋白质的肺水肿。水通道蛋白可能在VILI中具有保护作用。(C)2001 Elsevier Science B. V.保留所有权利。
Adult Respiratory Distress Syndrome is a disease with functional lung heterogeneity and thus a ventilator-delivered breath may over-distend non-involved areas. In rats we examined ventilator-delivered tidal volume (TV) breaths of 7 and 20 ml/kg on lung water as evidence of lung injury. We examined the role of aquaporins on ventilator-induced lung injury (VILI) by infusing HgCl2 which inhibits aquaporins by binding cysteine. Wet to dry lung weight ratio (W/D) as evidence of lung water was 4.47 +/- 0.1 SEM in controls, 4.6 +/- 0.1 and 5.5 +/- 0.2 (P < 0.05) in rats ventilated at 7 and 20 ml/kg, respectively. Pulmonary artery pressure (PAP) rose from 23 +/- 1 to 26 +/- 1 mmHg (P < 0.05, n = 7) and cardiac output fell from 104 +/- 2 to 67 +/- 3 ml/min (P < 0.05) in rats ventilated at 30 ml/kg. Left ventricular end diastolic pressure (n = 3) was unchanged. Evans Blue dye, an albumin marker, increased from a control 37 +/- 11 to 97 +/- 41 mg/g wet lung in TV 20 rats (P < 0.05). HgCl2 infused slowly by tail vein did not significantly raise PAP, but did increase W/D to 6 +/- 0.2 (P < 0.05) in rats ventilated at 20 ml/kg but not at 7 ml,kg. Equimolar cysteine: infusions prevented the HgCl2 from increasing the W/D above that seen with TV 20 ml/kg. Thus ventilation with TV of 20 ml/kg produced a protein-rich lung edema. Aquaporin channels may have a protective effect in VILI. (C) 2001 Elsevier Science B.V. All rights reserved.