Neuron-specific and inducible recombination by Cre recombinase in the mouse
Neuron-specific and inducible recombination by Cre recombinase in the mouse
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DOI：
10.1097/wnr.0b013e3282fb7d99
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发表时间：
2008-04-16
期刊：
影响因子：
1.7
通讯作者：
Harada, Akihiro
中科院分区：
文献类型：
--
作者：
Moto, Yukiko Hashi;Muramatsu, Kazuhiro;Harada, Akihiro
为了研究体内基因的神经元功能，需要神经元特异性和可诱导的基因靶向系统。在这项研究中，我们构建了一个敲入小鼠品系，该品系在神经元中表达由 Cre 重组酶和孕酮受体 (CrePR) 组成的融合蛋白。 CrePR 的神经元特异性表达是通过将 CrePR 基因插入 tau 位点来实现的，因为 T 在神经元中强烈表达，但在胶质细胞和其他组织中很少表达。通过将该敲入小鼠系 (tau(CrePR)) 与 ROSA26 lacZ 报告小鼠系 (R26R) 杂交，我们观察到抗孕酮 RU486 可以特异性地在神经元中诱导 CrePR 的重组酶活性。因此，tau(CrePR) 敲入系是研究神经元基因功能的有用工具。
To investigate the neuronal function of genes in vivo, a neuron-specific and inducible gene targeting system is desirable. In this study, we generated a knockin mouse line that expresses a fusion protein consisting of the Cre recombinase and the progesterone receptor (CrePR) in neurons. The neuron-specific expression of CrePR was attained by inserting CrePR gene into the tau locus, because T is expressed strongly in neurons but scarcely in glias and other tissues. By crossing this knockin mouse line (tau(CrePR)) with ROSA26 lacZ reporter mouse line (R26R), we observed that the anti progesterone RU486 could induce recombinase activity of the CrePR specifically in neurons. Thus, tau(CrePR) knockin line is a useful tool for studying neuronal gene functions.