Quorum sensing modulates transcription of cpsQ-mfpABC and mfpABC in Vibrio parahaemolyticus
Quorum sensing modulates transcription of cpsQ-mfpABC and mfpABC in Vibrio parahaemolyticus
复制标题
群体感应调节副溶血弧菌中 cpsQ-mfpABC 和 mfpABC 的转录。
DOI：
10.1016/j.ijfoodmicro.2013.07.008
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发表时间：
2013-09-16
影响因子：
5.4
通讯作者：
Mao, Panyong
中科院分区：
文献类型：
--
作者：
Zhou, Dongsheng;Yan, Xiaojuan;Mao, Panyong
副溶血性弧菌AphA和OpaR是群体感应（quorum sensing，QS）的两个主要调节因子，它们分别在低细胞密度（LCD）和高细胞密度（HCD）下大量产生并起作用，结果是这两种蛋白质的梯度产生，并在LCD和HCD之间过渡。cpsQ-mfpABC基因簇在副溶血性弧菌中转录为两个操纵子cpsQ-mfpABC和mfpABC。MfpABC是一种推定的膜融合转运蛋白，有助于生物膜的发展。CpsQ是一种c-di-GMP结合调节剂，可激活荚膜多糖基因和mfpABC的表达，从而诱导生物膜发育。如本研究所示，OpaR和AphA分别与mfpABC的启动子区域结合以增强和抑制其转录。相比之下，AphA和OpaR分别对cpsQ-mfpABC的正调节和负调节可能通过AphA或OpaR作用于cpsQ-mfpABC的另外的未知调节物来实现。由于OpaR和AphA的上述相互调节作用，cpsQ-mfpABC和mfpABC的转录水平随着LCD向HCD的转变而逐渐增强。这里提供的数据提出了一种新的模式，在控制QS调节子成员的表达的两个主QS调节剂的联合行动。(C)2013年由Elsevier B. V.出版
Vibrio parahaemolyticus AphA and OpaR are the two master regulators of quorum sensing (QS) that are abundantly produced and operate at low cell density (LCD) and high cell density (HCD), respectively, with an outcome of reciprocally gradient production of these two proteins with transition between LCD and HCD. The cpsQ-mfpABC gene cluster is transcribed as two operons cpsQ-mfpABC and mfpABC in V. parahaemolyticus. MfpABC is a putative membrane fusion transporter that contributes to biofilm development. CpsQ is a c-di-GMP-binding regulator that activates the expression of capsular polysaccharide genes and mfpABC and, thus, induces biofilm development. As shown in this study, OpaR and AphA bind to the promoter region of mfpABC to enhance and repress its transcription, respectively. In contrast, the positive and negative regulation of cpsQ-mfpABC by AphA and OpaR, respectively, achieves probably through acting of AphA or OpaR on additional unknown regulator(s) of cpsQ-mfpABC. The transcriptional levels of cpsQ-mfpABC and mfpABC enhance gradually with transition from LCD to HCD due to the above reciprocal regulatory action of OpaR and AphA. Data presented here present a novel paradigm of combined action of the two master QS regulators in controlling expression of the QS regulon members. (C) 2013 Published by Elsevier B.V.